AM26775PU-N Mouse IgG2a

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0.1 mg / €190.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Mouse IgG2a MOPC-173


Product Description for Mouse IgG2a

Mouse anti Mouse IgG2a MOPC-173.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry.

Properties for Mouse IgG2a

Product Category Isotype Controls
Quantity 0.1 mg
Presentation Purified
Applications F, ICC/IF, WB
Clonality Monoclonal
Clone MOPC-173
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Mineral oils
Flow cytometry.
Western blot.
Control experiments.
The reagent is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background.
Background The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-173, generated against an undefined antigen, does not react specifically with mouse, rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Concentration 1.0 mg/ml
General Readings
  1. Fougereau M, Bourgois A, de Preval C, Rocca-Serra J, Schiff C. The complete sequence of the murine monoclonal immunoglobulin MOPC 173 (IgG2a): genetic implications. Ann Immunol (Paris). 1976 Sep-Oct;127(5):607-31. PubMed PMID: 984731.
  2. Baumal R, Scharff MD. Immunoglobulin biosynthesis by the MOPC 173 mouse myeloma tumor and a variant spleen clone. J Immunol. 1976 Jan;116(1):65-74. PubMed PMID: 812916.
  3. Gupta V, Gylling A, Alonso JL, Sugimori T, Ianakiev P, Xiong JP, et al. The beta-tail domain (betaTD) regulates physiologic ligand binding to integrin CD11b/CD18. Blood. 2007 Apr 15;109(8):3513-20. Epub 2006 Dec 14. PubMed PMID: 17170130. (Free PMC Article available, 7 images available)
  4. Khoddami V, Cairns BR. Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP. Nat Protoc. 2014 Feb;9(2):337-61. doi: 10.1038/nprot.2014.014. Epub 2014 Jan 16. PubMed PMID: 24434802.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
Buffer System:
Phosphate buffered saline (PBS)
15 mM sodium azide, approx. pH 7.4
Liquid Ig fraction
Species Reactivity
Species reactivity (tested):
Negative on Human, Mouse, and Rat
This antibody reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.

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