AM03096D5-N Mouse IgG2a Isotype Control

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Mouse anti Mouse IgG2a Isotype Control PPV-04


Product Description for Mouse IgG2a Isotype Control

Mouse anti Mouse IgG2a Isotype Control PPV-04.
Presentation: DY547
Product is tested for Flow Cytometry.

Properties for Mouse IgG2a Isotype Control

Product Category Isotype Controls
Quantity 0.1 mg
Synonyms Mouse IgG2a Negative Control
Presentation DY547
Applications F
Clonality Monoclonal
Clone PPV-04
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Application The reagent is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background.
Background The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay.
Concentration 0.1 mg/ml
Storage Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Conjugated with Dyomics 547 under optimum conditions. The conjugate is purified by size-exclusion chromatography
Buffer System:
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Liquid purified Ig fraction
Species Reactivity
Species reactivity (tested):
Plant. Negative species: Human, Porcine, Mouse, Rat; other species expected negative.
This antibody antibody (clone PPV-04) reacts with undefined epitope on a plant pathogen.
It does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

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