AM03096PU-N Mouse IgG2a Isotype Control

Search for all "Mouse IgG2a Isotype Control"

0.1 mg / €190.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Mouse IgG2a Isotype Control PPV-04


Product Description for Mouse IgG2a Isotype Control

Mouse anti Mouse IgG2a Isotype Control PPV-04.
Presentation: Purified
Product is tested for Flow Cytometry.

Properties for Mouse IgG2a Isotype Control

Product Category Isotype Controls
Target Category
Quantity 0.1 mg
Presentation Purified
Applications F
Clonality Monoclonal
Clone PPV-04
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Add. information Usage: The reagent is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Application Flow Cytometry.
Control Experiments: The antibody is suitable for control experiments when performing cell surface staining as well as intracellular staining.
Background The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 µg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a Negative Control for each specific monoclonal antibody used in a particular immunoassay.
Concentration 1.0 mg/ml
Product Citations
Purchased from Acris:
1. MacKenzie B, Korfei M, Henneke I, Sibinska Z, Tian X, Hezel S, et al. Increased FGF1-FGFRc expression in idiopathic pulmonary fibrosis. Respir Res. 2015 Jul 3;16:83. doi: 10.1186/s12931-015-0242-2. PubMed PMID: 26138239
2. Sandetskaya, N;Moos, D;Pötter, H;Seifert, S;Jenerowicz, M;Becker, H;Kuhlmeier, CZ. An integrated versatile lab-on-a-chip platform for the isolation and nucleic acid-based detection of pathogens. Future Science OA 2017.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Affinity Chromatography on Protein A
Buffer System:
PBS, pH~7.4
15mM Sodium Azide
Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
This Mouse IgG2a monoclonal antibody (clone PPV-04) reacts with undefined epitope on a plant pathogen.
Negative Species: Human, Porcine, Mouse, Rat, other species Not tested (expected Negative).
The monoclonal antibody PPV-04, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an Mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

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