AM08216PU-N Mouse IgG2c Isotype Control

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1 mg / €380.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Mouse IgG2c Isotype Control 6.3


Product Description for Mouse IgG2c Isotype Control

Mouse anti Mouse IgG2c Isotype Control 6.3.
Presentation: Purified
Product is tested for Enzyme Immunoassay.

Properties for Mouse IgG2c Isotype Control

Product Category Isotype Controls
Quantity 1 mg
Synonyms Mouse IgG2c Negative Control
Presentation Purified
Applications E
Clonality Monoclonal
Clone 6.3
Host Mouse
Isotype IgG2c
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Application ELISA: Working dilution  1 µg/ml with unlabeled mouse (C57BL/6) IgG2c antibodies. 
See also Ref.1-5.
Background Isotype Control monoclonal antibodies are used to estimate the non-specific binding of target primary antibodies to cell surface antigens. Non-specific binding is due to Fc receptor binding or other protein-protein interactions. Isotype controls show negligible cross-reactivity with cell surface antigens on tissue sections or in cellular preparations. Isotype Controls should be used at identical concentrations and staining conditions as the target primary antibodies.
Concentration 1.0 mg/ml
General Readings
  1. Haas KM, Watanabe R, Matsushita T, Nakashima H, Ishiura N, Okochi H, et al. Protective and pathogenic roles for B cells during systemic autoimmunity in NZB/W F1 mice. J Immunol. 2010 May 1;184(9):4789-800. doi: 10.4049/jimmunol.0902391. Epub 2010 Apr 5. PubMed PMID: 20368280. (Free PMC Article available, 6 images available)
  2. Allan RS, Zueva E, Cammas F, Schreiber HA, Masson V, Belz GT, et al. An epigenetic silencing pathway controlling T helper 2 cell lineage commitment. Nature. 2012 Jul 12;487(7406):249-53. doi: 10.1038/nature11173. PubMed PMID: 22763435.
  3. Donius LR, Handy JM, Weis JJ, Weis JH. Optimal germinal center B cell activation and T-dependent antibody responses require expression of the mouse complement receptor Cr1. J Immunol. 2013 Jul 1;191(1):434-47. doi: 10.4049/jimmunol.1203176. Epub 2013 Jun 3. PubMed PMID: 23733878. (Free PMC Article available, 11 images available)
  4. Vogelzang A, McGuire HM, Liu SM, Gloss B, Mercado K, Earls P, et al. IL-21 contributes to fatal inflammatory disease in the absence of Foxp3+ T regulatory cells. J Immunol. 2014 Feb 15;192(4):1404-14. doi: 10.4049/jimmunol.1302285. Epub 2014 Jan 20. PubMed PMID: 24446516.
  5. Pioli PD, Chen X, Weis JJ, Weis JH. Fatal autoimmunity results from the conditional deletion of Snai2 and Snai3. Cell Immunol. 2015 May;295(1):1-18. doi: 10.1016/j.cellimm.2015.02.009. Epub 2015 Feb 24. PubMed PMID: 25732600.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Buffer System:
100 mM Borate buffered saline, pH 8.2.
No preservatives or amine-containing buffer salts added.
Liquid purified Ig fraction.

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