AM03111PU-N Mouse IgM Isotype Control

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0.1 mg / €190.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Mouse anti Mouse IgM Isotype Control PFR-03

AM03111PU-N

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  • AM03111PU-N

Product Description for Mouse IgM Isotype Control

Mouse anti Mouse IgM Isotype Control PFR-03.
Presentation: Purified
Product is tested for Flow Cytometry.

Properties for Mouse IgM Isotype Control

Product Category Isotype Controls
Target Category
Quantity 0.1 mg
Presentation Purified
Applications F
Clonality Monoclonal
Clone PFR-03
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Isotype control SM13P
Application Flow Cytometry.
Control Experiments: The antibody is suitable for control experiments when performing cell surface staining as well as intracellular staining. 
Recommended Dilutions: The reagent is intended as Isotype Control for Flow Cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this Isotype Control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the Isotype Control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg per ml of incubation mixture), change the conditions of your experiment to reduce the background.
Background The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PFR-03, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgM molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Concentration 1.0 mg/ml
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Precipitation Methods
Buffer System:
Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.
State:
Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
Purified
Specificity
Specificity:
This Mouse IgM monoclonal antibody (clone PFR-03) reacts with undefined epitope on a plant pathogen.
Negative Species: Human, Porcine, Mouse, Rat, Other species Not tested (expected Negative).

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