Product Description for Mouse IgM Isotype Control
Mouse anti Mouse IgM Isotype Control PFR-03.
Presentation: PE
Product is tested for Flow Cytometry.
Presentation: PE
Product is tested for Flow Cytometry.
Properties for Mouse IgM Isotype Control
| Product Category | Isotype Controls |
|---|---|
| Quantity | 0.1 mg |
| Presentation | PE |
| Applications | F |
| Clonality | Monoclonal |
| Clone | PFR-03 |
| Host | Mouse |
| Isotype | IgM |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Application | The reagent is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background. |
|---|---|
| Background | The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PFR-03, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgM molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished. |
| Concentration | 0.1 mg/ml |
| Storage | Store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Shelf life: one year from despatch. |
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Accessory Products
Recommended Secondary Antibodies for Mouse IgM Isotype Control (10 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1349AP |
Mouse IgA (H+L chain) antibody |
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|
Goat | AP | |||||
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| R1349B |
Mouse IgA (H+L chain) antibody |
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|
|
Goat | Biotin | |||||
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| R1349F |
Mouse IgA (H+L chain) antibody |
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|
|
Goat | FITC | |||||
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| R1349HRP |
Mouse IgA (+IgG +IgM) antibody |
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|
|
Goat | HRP | |||||
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| R1349T |
Mouse IgA (H+L chain) antibody |
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|
|
Goat | TRITC | |||||
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| R1349TR |
Mouse IgA (H+L chain) antibody |
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|
|
Goat | Texas Red | |||||
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| SP1018AP |
Mouse IgM (Mu chain specific), adsorbed antibody |
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|
|
Goat | AP | Ms | ||||
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| SP1018F |
Mouse IgM (Mu chain specific), adsorbed antibody |
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|
|
Goat | FITC | Ms | ||||
| Acris Antibodies GmbH | |||||||
| SP1018HRP |
Mouse IgM (Mu chain specific), adsorbed antibody |
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|
|
Goat | HRP | Ms | C, E, P | |||
| Acris Antibodies GmbH | |||||||
| SP1018R |
Mouse IgM (Mu chain specific), adsorbed antibody |
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|
Goat | PE | Ms | ||||
| Acris Antibodies GmbH | |||||||
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