Primary Antibodies

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MYB antibody

Western blot using affinity purified anti-C-Myb antibody in Cos7 cell lysates: Lane 1: vector; Lane 2: transfected with c-myb, no treatment; Lane 3: transfected with c-Myb and heat stress-treated (incubation at 42οC for 30 minutes) to induce c-Myb modification. Lane 3 shows detection of bands at 75, 98, and 125kDa corresponding to overexpressed c-Myb (arrows): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively. Nitrocellulose 0.45µm membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS. The primary antibody was used at 1:500. Rabbit IgG Aff - Purified Bonobo, Chimp, Eq, Gorilla, Hu, Mky, Ms, Op, Orang-Utan, Por, Rt E, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

MYB antibody

Western blot using affinity purified anti-C-Myb antibody in Cos7 cell lysates: Lane 1: vector; Lane 2: transfected with c-myb, no treatment; Lane 3: transfected with c-Myb and heat stress-treated (incubation at 42οC for 30 minutes) to induce c-Myb modification. Lane 3 shows detection of bands at 75, 98, and 125kDa corresponding to overexpressed c-Myb (arrows): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively. Nitrocellulose 0.45µm membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS. The primary antibody was used at 1:500. Rabbit IgG Aff - Purified Bonobo, Chimp, Eq, Gorilla, Hu, Mky, Ms, Op, Orang-Utan, Por, Rt E, WB
25 µl / €190.00
  Acris Antibodies GmbH

SMAD1 antibody

  Rabbit polyclonal anti-SMAD1 antibody  
WB using Anti-SMAD1 antibody shows detection of endogenous SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) and keratinocyte (HaCaT, lanes 5-8) derived cell lines treated with PBS, BMP2, EGF, or NaCl for 1 h at 37? before harvest. Each lane contains approximately 15 µg of lysate. Primary antibody was used at 1:500. Anti-beta actin staining was used as a loading control. The membrane was washed and reacted with a 1:3,000 dilution HRP-conjugated a-Rabbit IgG. Rabbit IgG Aff - Purified Bonobo, Can, Cattle, Chimp, Chk, Dros, Duckbill platypus, Eq, Gorilla, Hu, Macaque, Ms, Op, Orang-Utan, Por, Rt, Salmon, Sh E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

SMAD1 pSer206 antibody

  Rabbit polyclonal SMAD1 phospho S206 antibody  
WB using Anti-SMAD1 pS206 antibody shows detection of endogenous phosphorylated SMAD1 in whole cell lysates from HEPG2 (lanes 1-4) derived cell lines treated with PBS, BMP2 (5ng/mL), EGF (1ng/mL), or NaCl for 1h at 37? before harvest. Primary antibody was used at 1:500 and pre-incubated before reacting with blot as follows: top row - with PBS,middle row - with the immunizing phosphorylated peptide and bottom row - with control or non-phosphorylated peptide. HRP-conjugated-Rabbit IgG was 1:3,000. Rabbit IgG Aff - Purified Bonobo, Can, Cattle, Chimp, Chk, Dros, Duckbill platypus, Eq, Gorilla, Hu, Macaque, Ms, Op, Orang-Utan, Por, Rt, Salmon, Sh E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

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