Primary Antibodies

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATG12 / APG12 antibody

Figure 1. Immunoblot of APG12 fusion protein. Anti-APG12 antibody generated by immunization with recombinant yeast APG12
was tested by immunoblot against yeast lysates expressing the APG12-GFP fusion protein and other UBL fusion proteins. All UBLs
possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody
against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing
recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX. Panel C shows specific
reaction with APG12 using a 1:2,000 dilution of IgG fraction of Rabbit-anti-APG12 (Yeast) followed by reaction with a
1:15,000 dilution of HRP Goat-a-Rabbit IgG MX. All primary antibodies were diluted in TTBS buffer supplemented
with 5% non-fat milk and incubated with the membranes overnight at 4° C. Yeast lysate proteins were separated by SDS-PAGE using a
15% gel. This data indicates that anti-APG12 is highly specific and does not cross react with other UBLs. A chemiluminescence
system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M. Malakhov,
Lifesensors Inc., personal communication. Rabbit Purified S. cerevisiae, Ye E, WB
0.5 mg / €510.00
  Acris Antibodies GmbH

CCT1 / TCP1 antibody

  Rat Monoclonal Anti-TCP1 alpha Antibody  
WB of human lysates showing detection of ~60  kDa TCP1-alpha  protein using Anti-TCP1 , clone 91a.  Lane 1: MW ladder. Lane 2: A431 lysate, 20 µg. Lane3: HEK293 lysate, 20µg. Block: 5% milk + TBST 1hr at RT. Primary antibody: Rat monoclonal Anti-TCP1-alpha , clone 91a incubated for 60 min at RT. Secondary antibody: Goat Anti-Rat HRP antibody (1:1000) for 60 min at RT. Colour Development: TMB solution for 5 min at RT. Rat IgG2a 91A Purified Bov, Can, Ce, Dros, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, S. cerevisiae WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Heat shock protein 70 / HSP70 antibody

  Mouse monoclonal Hsp70 Antibody  
Western blot analysis of Hsp70 in rat tissue lysates, using a dilution of the antibody Mouse IgG1 5A5 Aff - Purified Amph, Av, Dros, Fi, Hu, Ms, Rb, Rt, S. cerevisiae ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Heat shock protein 70 / HSP70 antibody

  Mouse monoclonal Hsp70 Antibody  
Western blot analysis of Hsp70 in rat tissue mix using a 1:5000 dilution of the antibody Mouse IgM 2A4 Purified Amph, Chk, Dros, Fi, Hu, Ms, Rb, Rt, S. cerevisiae C, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Heat shock protein 70 / HSP70 antibody

  Mouse monoclonal Hsp70 Antibody  
10ug cell free extract from Artemia probed with anti-Hsp70 Mouse IgG1 3A3 Aff - Purified Amph, Chk, Dros, Fi, Hu, Ms, Rb, Rt, S. cerevisiae ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Heat Shock Protein 90 (HSP90) alpha/beta antibody

  Mouse Monoclonal anti-HSP90AA1 Antibody  
Western blot analysis of Hsp90 in cell lysates from 12 rat tissue lines at a 1:1000 dilution of TA309402. Mouse IgG2a Hyb-K41220A Purified Hu, Rt, S. cerevisiae, S.pombe C, E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HIRA / TUPLE1 antibody

Figure 1. Western blot analysis using TUP1 antibody (AM05331PU-N) on recombinant TUP1 protein (amino acids 1-200) expressed in Mav108 cells. Mouse IgG 10 Purified S. cerevisiae WB
0.1 mg / €370.00
  Acris Antibodies GmbH

HSP31p (Yeast, 50-64) antibody

HSP31 Antibody Cat.-No AP32062PU-N (0.05 µg/ml) staining of Yeast lysate (wt) and the KO (delta) in the left panel and the Ponceau stain in the right panel (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained by Dr. M. Skoneczny, IBB, Warsaw, Poland. Goat Aff - Purified S. cerevisiae E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

IGL antibody

  Mouse monoclonal Anti-Lambda light chain Clone N10/2  
IGL Mouse IgG1 N10/2 Purified Hu, S. cerevisiae P
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCM2 (21-31) antibody

  Rabbit polyclonal anti-MCM2 antibody  
WB using anti-MCM2 antibody shows detection of both phosphorylated and unphosphorylated MCM2 in nuclear extracts from elutriated human cells(MO59K/K562). Panel A shows WB results for asynchronous cells lyastes(lane 1), cells arrested in early S with aphidicolin (lane2), and cells arrested in mitosis with nocodazole (lane 3). Panel B shows a schematic diagram of bands representing phosphorylated and unphosphorylated MCM2 present in these preparations. The primary antibody was diluted 1:400. Rabbit IgG Aff - Purified Hu, Ms, Rt, S. cerevisiae E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

MER2 pSer30 antibody

Western blot using affinity purified anti-S.cerevisiae Mer2 pS30 antibody shows detection of phosphorylated Mer2 but not phosphatase treated or mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first  immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody appears to be specific for phosphorylated Mer2 at the S30 position with negligible cross reactivity against unphosphor-ylated protein. The primary antibody was used at a 1:5,000 dilution. Rabbit IgG Aff - Purified S. cerevisiae E, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

MER2 (26-35) antibody

Western blot using affinity purified anti-S.cerevisiae Mer2 antibody shows detection of phosphorylated and unphosphorylated Mer2 in wild type, phosphatase treated and mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody is reactive with both phosphor-ylated and unphosphorylated Mer2 at the S30 position. The primary antibody was used at a 1:5,000 dilution. Rabbit IgG Aff - Purified S. cerevisiae E, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

MRE11 (578-590) antibody

  Rabbit polyclonal anti-Mre11 antibody  
ChIP using Mre11 (S. cerevisiae) antibody. Chromatin was immunoprecipitated with the antibody at the stated dilutions. Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus). Rabbit IgG Aff - Purified S. cerevisiae ChIP, E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

NUP84 (N-term) antibody

AP31409PU-N NUP84 antibody staining of Saccharomyces cerevisiae S288c lysate at 2 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Dr. F Reggiori, University of Utrecht, Netherlands. Goat Aff - Purified S. cerevisiae E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

POLR2A antibody

  Mouse monoclonal Anti-RNA polII Clone CTD 4H8  
POLR2A Mouse IgM CTD 4H8 Purified Hu, S. cerevisiae ChIP, E, F, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PTGES3 / TEBP antibody

  Mouse monoclonal p23 Antibody  
Western blot analysis of p23 in a human cell line mix at a 1:1000 dilution of the antibody Mouse IgG1 JJ6 Purified Chk, GP, Hu, Ms, Rb, S. cerevisiae E, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAD23B (163-176) antibody

  Goat polyclonal anti-HR23B antibody  
WB using Anti-HR23B antibody shows detection of a band at ~58 kDa (arrowhead) corresponding to HR23B present in a HeLa whole cell lysate.  Pre-incubation of antibody with immunizing peptide completely blocks reactivity (data not shown).   Primary antibody diluted to 1:500 in 5% BLOTTO in PBS. The membrane was washed and reacted with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] for 45 min at RT. Goat IgG Aff - Purified Can, Chimp, Hu, S. cerevisiae E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

YDL126C / VCP (Baker's Yeast, 128-142) antibody

  Goat Anti-CDC48/ VCP / YDL126C (baker's yeast, aa128-142) Polyclonal Antibody  
TA329667 (0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt, S. cerevisiae E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

YDL126C / VCP antibody

  Goat Anti-CDC48 / VCP / YDL126C Polyclonal Antibody  
TA329668 (0.01ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt, S. cerevisiae E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

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