Primary Antibodies

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

6xHistidine Epitope Tag (HHHHHH) antibody

Western blot analysis of His-Azami-Green (1) and His-EGFP (2) using AM26687AF-N. Mouse IgG1 6C4 Azide Free WB
0.1 mg / €350.00
  OriGene Technologies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

6xHistidine Epitope Tag Chicken Ig Purified WB
0.4 ml / €420.00
  OriGene Technologies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

6xHistidine Epitope Tag Chicken Ig Aff - Purified IP
0.1 ml / €420.00
  OriGene Technologies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

6xHistidine Epitope Tag Rabbit Serum E, WB
0.2 ml / €350.00
  OriGene Technologies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Western blot (AP) of several His-tagged proteins  from supernatants (A) and after purification (B) by affinity chromatography using metal chelate columns (Ni2+, Co2+) as matrix. The anti-His-tag antibody concentration used was 0.1µg/ml. Rabbit Aff - Purified WB
50 µg / €210.00
  OriGene Technologies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Figure 1. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. The blot was blocked with 3% BSA in TBST for 45 min at RT. Antibody was incubated with blot at a 1/1,000 dilution in TBST with 3% BSA for 1 hour at RT. Detection occurred using HRP Goat-anti-Rabbit IgG  diluted 1/80,000 in blocking buffer for 30 min at RT. Lane 1 was loaded with 12-Epitope Tag Protein Marker Lysate which has the His epitope tag incorporated through a C-terminal linkage (~42 kDa). Lane 2 was loaded with His-SUMO-GFP recombinant protein which has the His epitope tag incorporated through an N-terminal linkage (~40 kDa). A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. Proteins were transferred to nitrocellulose using standard methods. Molecular weights were estimated by comparison to standards (lane M). Rabbit Aff - Purified C, E, WB
0.1 mg / €320.00
  OriGene Technologies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 µg/ml was used to detect 1.0 µg of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 30 min at room temperature. LICORs Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Rabbit Biotin C, E, WB
0.1 mg / €460.00
  OriGene Technologies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 µg/ml was used to detect 1.0 µg of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 30 min at room temperature. LICORs Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Rabbit HRP C, E, WB
0.1 mg / €460.00
  OriGene Technologies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualised with FITC conjugated Mouse anti-HIS tag antibody (SM1693F). Mouse IgG1 AD1.1.10 FITC F, ICC/IF
0.1 mg / €450.00
  OriGene Technologies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Western blot analysis of His-Ubiquitin recombinant protein probed with MOUSE ANTI HISTIDINE TAG:HRP (SM1693HRP). Mouse IgG1 AD1.1.10 HRP E, WB
0.1 mg / €600.00
  OriGene Technologies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag antibody Cat.-No SM1693P Mouse IgG1 AD1.1.10 Purified C, E, IP, WB
1 mg / €620.00
  OriGene Technologies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Immunofluorescence staining of His Tag fusion protein in 293 cells using His tag antibody.
A: untransfected Control 
B: Transfected Mouse IgG2b HIS.H8 Aff - Purified All., Mam ICC/IF, IP, WB
50 µg / €270.00
  OriGene Technologies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

6xHistidine Epitope Tag Mouse IgG1 His17 Purified E, IP, WB
1 mg / €330.00
  HyTest Ltd.

7-DHC reductase antibody

  Rabbit Polyclonal Anti-DHCR7 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (DHCR7 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

8-Hydroxy-Guanosine / 8-OHG antibody

AM03160PU used against oxidized 8-OH-dG in ischemic Rat brain tissue (Left Panel). 
Center Panel: DAPI staining. 
Right Panel: merged. 
Courtesy of Dr. Yang, University of New Mexico, USA. Mouse IgG2b 15A3 Purified C, Dot, E, ICC/IF, P
0.1 mg / €360.00
  OriGene Technologies GmbH

8-Hydroxy-Guanosine / 8-OHG antibody

AM03160PU used against oxidized 8-OH-dG in ischemic Rat brain tissue (Left Panel). 
Center Panel: DAPI staining. 
Right Panel: merged. 
Courtesy of Dr. Yang, University of New Mexico, USA. Mouse IgG2b 15A3 Purified C, Dot, E, ICC/IF, P
25 µg / €210.00
  OriGene Technologies GmbH

8-Hydroxy-Guanosine / 8-OHG antibody

Immunohistochemistry: AM20226PU 8-OHdG antibody staining of Paraffin Section of  UV-irradiated Mouse Skin using an antigen retrieval  in 0.1% Trypsin. The section was incubated with AM20226PU (5 µg/ml), followed by staining with Avidin-Biotin-Complex method. This antibody stains epidremal cells and some infiltrating neutrophils and macrophages.
(See Ref.3 for more details). Mouse IgG1 N45.1 Purified Hu, Ms, Rb, Rt E, ICC/IF, P
20 µg / €490.00
  OriGene Technologies GmbH

8-Hydroxy-Guanosine / 8-OHG antibody

8-Hydroxy-Guanosine / 8-OHG Mouse IgG2b 15A3 Purified C, E, ICC/IF
0.1 mg / €650.00
  OriGene Technologies GmbH

8-Hydroxy-Guanosine / 8-OHG antibody

8-Hydroxy-Guanosine / 8-OHG Goat Serum C, E, P
0.2 ml / €390.00
  OriGene Technologies GmbH

8-Hydroxy-Guanosine / 8-OHG antibody

8-Hydroxy-Guanosine / 8-OHG Goat Serum E, P
0.1 ml / €410.00
  OriGene Technologies GmbH

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