Primary Antibodies

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

6xHistidine Epitope Tag (HHHHHH) antibody

Western blot of HIS-tagged protein probed with Mouse anti Histidine tag (SM1693P/PX) visualised with Rabbit anti Mouse IgG -HRP conjugated (SP1012HRP). Mouse IgG1 AD1.1.10 Purified C, E, IP, WB
0.1 mg / €250.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Western blot of HIS tagged protein probed with mouse anti Histidine Tag - Biotin (SM1693B) and visualised with Streptavidin - HRP Mouse IgG1 AD1.1.10 Biotin C, E, IP, WB
0.1 mg / €610.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Western blot of HIS-tagged protein probed with Mouse anti Histidine tag (SM1693P/PX) visualised with Rabbit anti Mouse IgG -HRP conjugated (SP1012HRP). Mouse IgG1 AD1.1.10 Purified C, E, IP, WB
2 mg / €1,250.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Immunofluorescence of His-tagged fusion protein expressed in 293 cells. Left Lane: Untransfected control; Right Lane: Transfected cells. Mouse IgG2b BV-G020 Aff - Purified ICC/IF, IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Figure 1. Western blot of His tagged protein (vector pET 30C) using AM05288PU-N antibody. Lane 1: Whole cellular extract of BL21 (DE3) pJT4pTT9 bacteria carrying gene encoding for His-Rel E protein (uninduced). Lane 2: Induced. Lane 3: Induced (1/2 dilution). Lane 4: Purified His-Rel E protein (~ 17 kDa). Mouse IgG2a IPA2C5.1 Purified WB
0.1 mg / €370.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Figure 1. HIS-tagged recombinant protein was expressed in E. Coli, and used to test the 6xHIS monoclonal antibody (6AT18). Mouse IgG1 6AT18 Ascites WB
0.4 ml / €370.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Immunofluorescence staining of His Tag fusion protein in 293 cells using His tag antibody.
A: untransfected Control 
B: Transfected Mouse IgG2b HIS.H8 Aff - Purified All., Mam ICC/IF, IP, WB
50 µg / €230.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

6xHistidine Epitope Tag Mouse IgG1 16-001 Aff - Purified WB
0.1 mg / €400.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Western Blot testing of anti His6 Monoclonal antobody. Production of recombinant, C-terminal Hexahistidine tag-containing IL-10 (23.2 kDa) was tested from CHO-based cell line supernatant. Mouse IgG2b 16-002 Aff - Purified E, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Western blotting using anti-His antibodies (HisH8 and 10A12) to detect His-tagged HIV p24 Mouse IgG1 10A12 Purified WB
0.5 mg / €420.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Western blotting using anti-His antibodies (HisH8 and 10A12) to detect His-tagged HIV p24 Mouse IgG1 10A12 Purified WB
0.1 mg / €290.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Western blot analysis of His-Azami-Green (1) and His-EGFP (2) using AM26687AF-N. Mouse IgG1 6C4 Azide Free WB
0.1 mg / €350.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

6xHistidine Epitope Tag Chicken Ig Purified WB
0.4 ml / €420.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

6xHistidine Epitope Tag Chicken Ig Aff - Purified IP
0.1 ml / €420.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

6xHistidine Epitope Tag Rabbit Serum E, WB
0.2 ml / €350.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Western blot (AP) of several His-tagged proteins  from supernatants (A) and after purification (B) by affinity chromatography using metal chelate columns (Ni2+, Co2+) as matrix. The anti-His-tag antibody concentration used was 0.1µg/ml. Rabbit Aff - Purified WB
50 µg / €210.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Figure 1. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. The blot was blocked with 3% BSA in TBST for 45 min at RT. Antibody was incubated with blot at a 1/1,000 dilution in TBST with 3% BSA for 1 hour at RT. Detection occurred using HRP Goat-anti-Rabbit IgG  diluted 1/80,000 in blocking buffer for 30 min at RT. Lane 1 was loaded with 12-Epitope Tag Protein Marker Lysate which has the His epitope tag incorporated through a C-terminal linkage (~42 kDa). Lane 2 was loaded with His-SUMO-GFP recombinant protein which has the His epitope tag incorporated through an N-terminal linkage (~40 kDa). A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. Proteins were transferred to nitrocellulose using standard methods. Molecular weights were estimated by comparison to standards (lane M). Rabbit Aff - Purified C, E, WB
0.1 mg / €310.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 µg/ml was used to detect 1.0 µg of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 30 min at room temperature. LICORs Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Rabbit Biotin C, E, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 µg/ml was used to detect 1.0 µg of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 30 min at room temperature. LICORs Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Rabbit HRP C, E, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

6xHistidine Epitope Tag (HHHHHH) antibody

Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualised with FITC conjugated Mouse anti-HIS tag antibody (SM1693F). Mouse IgG1 AD1.1.10 FITC F, ICC/IF
0.1 mg / €440.00
  Acris Antibodies GmbH

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