AP05569PU-N 14-3-3 protein beta+zeta pSer185 antibody

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0.1 mg / €450.00
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Quick Overview

Sheep anti Bovine, Human, Mouse, Rabbit, Rat, Sheep 14-3-3 protein beta+zeta

Product Description for 14-3-3 protein beta+zeta

Sheep anti Bovine, Human, Mouse, Rabbit, Rat, Sheep 14-3-3 protein beta+zeta.
Properties: pSer185
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for 14-3-3 protein beta+zeta

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms YWHAB, YWHAZ
Presentation Purified
Reactivity Bov, Hu, Ms, Rb, Rt, Sh
Applications WB
Clonality Polyclonal
Host Sheep
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide corresponding to 14-3-3 beta and zeta phosphorylated at serine 185
AA Sequence:
EILNSPEKAC
Property pSer185
Application Western Blot: 1/100-1/300. Detects a band of approximately 30kDa. 
Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Concentration 1.0 mg/ml
General Readings
  1. Aitken A, Howell S, Jones D, Madrazo J, Patel Y. 14-3-3 alpha and delta are the phosphorylated forms of raf-activating 14-3-3 beta and zeta. In vivo stoichiometric phosphorylation in brain at a Ser-Pro-Glu-Lys MOTIF. J Biol Chem. 1995 Mar 17;270(11):5706-9. PubMed PMID: 7890696.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (expected):
Mammals.
Species reactivity (tested):
Human, Bovine, Mouse, Rat, Rabbit, Sheep.
Specificity
Specificity:
This antibody recognizes the 14-3-3 isoforms beta and zeta which are phosphorylated at Serine 185. The members of the 14-3-3 family of proteins are highly conserved and are involved in multiple signalling pathways. The phosphorylated forms of beta and zeta 14-3-3 are found endogenously in brain tissue.
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