AP05567SU-N 14-3-3 protein gamma antibody

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0.1 ml / €470.00
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Quick Overview

Rabbit anti Bovine, Chicken, Human, Mouse, Rat, Sheep 14-3-3 protein gamma

Product Description for 14-3-3 protein gamma

Rabbit anti Bovine, Chicken, Human, Mouse, Rat, Sheep 14-3-3 protein gamma.
Presentation: Serum
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot, Paraffin Sections.

Properties for 14-3-3 protein gamma

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms KCIP-1, Protein kinase C inhibitor protein 1, YWHAG
Presentation Serum
Reactivity Bov, Chk, Hu, Ms, Rt, Sh
Applications E, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P61981
Immunogen:
Synthetic peptide of acetylated N-terminal amino acids 1-10 of sheep 14-3-3 gamma
AA Sequence:
Ac.VDREQLVQKAC
GeneID:
7532
Remarks:
Antiserum Preparation: Antisera to sheep 14-3-3 gamma were raised by repeated immunisations of rabbit with highly purified antigen.
Application ELISA.
Western Blot: 1/6000-1/12000; detects a band of approximately 30kDa in HEK293 cell lysates.
Immunohistochemistry on Paraffin Sections: 1/800.
Background 14-3-3 gamma is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.
14-3-3 gamma is thought to play an important role in muscle, being induced by growth factors in human vascular smooth muscle cells, and being expressed in skeletal and heart muscles. An example is the gamma isoform binds musclespecific receptor tyrosine kinase regulating synaptic gene transmission. The gamma isoform binds amyloid betaprotein precursor intracellular domain fragment (AICD) and FE65, facilitating FE65dependent gene transactivation. The binding of 14-3-3 gamma to glial fibrillary acidic protein is important in the regulation of glial filaments. 14-3-3 gamma binds and may regulate CDK11 during the cell cycle and apoptosis. The gamma isoform is lowered in foetal Downs syndrome brains.
General Readings 1. Martin, H. et al. (1993) Antibodies against major brain isoforms of 14-3-3 protein. An antibody specific for the Nacetylated aminoterminus of a protein. FEBS 331: 296 - 303.
2. Aitken, A. et al. (2006) 14-3-3 proteins: A historic overview. Seminars in Cancer Biology 16: 162 - 172.
3. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3ß and e are independent prognostic factors for these tumors.
PLoS One. 6: e24843.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
State:
Liquid Serum containing 0.09% Sodium Azide as preservative
Serum
Species Reactivity
Species reactivity (expected):
Mammals.
Species reactivity (tested):
Sheep, Mouse, Human, Bovine, Chicken, Rat.
Specificity
Specificity:
This antibody recognises the acetylated N-terminal of 14-3-3 gamma.
Gene ID 7532
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for 14-3-3 protein gamma (10 products)

Catalog No. Species Pres. Purity   Source  

14-3-3 protein gamma

14-3-3 protein gamma Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

14-3-3 protein gamma (1-247)

14-3-3 protein gamma Human Purified ≥ 95 by SDS-PAGE E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

14-3-3 protein gamma (1-247)

14-3-3 protein gamma Human Purified ≥ 95 by SDS-PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

14-3-3 protein gamma

14-3-3 protein gamma Human in vitro transl.
  Abnova Taiwan Corp.

14-3-3 protein gamma

14-3-3 protein gamma Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

14-3-3 protein gamma

14-3-3 protein gamma Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

14-3-3 protein gamma

14-3-3 protein gamma Human Purified
  Abnova Taiwan Corp.

14-3-3 protein gamma

14-3-3 protein gamma Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

14-3-3 protein gamma

14-3-3 protein gamma Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

14-3-3 protein gamma

SDS-Page: 14-3-3 gamma Protein [NBC1-18391] - 14-3-3 gamma, 28 kDa ( 247 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE Protein
  Novus Biologicals Inc.

Positive controls for 14-3-3 protein gamma (1 products)

Catalog No. Species Pres. Purity   Source  

14-3-3 gamma Lysate

Western Blot: 14-3-3 gamma Lysate [NBL1-17949] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for YWHAG
  Novus Biologicals Inc.
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