TA302401 14-3-3 protein sigma / SFN antibody

Goat Polyclonal Antibody against 14-3-3 sigma / Stratifin

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0.1 mg / €360.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Goat anti Canine, Human, Mouse, Rat 14-3-3 protein sigma / SFN

TA302401

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  • TA302401

Product Description for 14-3-3 protein sigma / SFN

Goat anti Canine, Human, Mouse, Rat 14-3-3 protein sigma / SFN.
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for 14-3-3 protein sigma / SFN

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Epithelial cell marker protein 1, HME1, Stratifin
Presentation Aff - Purified
Reactivity Can, Hu, Ms, Rt
Applications P, WB
Clonality Polyclonal
Host Goat
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P31947
Immunogen:
Peptide with sequence from the internal region of the protein sequence according to NP_006133.1.
AA Sequence:
C-DLHTLSEDSYKDST
GeneID:
2810
Application Peptide ELISA: 1/16000 (Detetion Limit). 
Western Blot: 0.03-0.1 µg/ml. Approx. 28kDa band observed in Human Skin lysates (calculated MW of 27.8kDa according to NP_006133.1).
Immunohistochemistry on Paraffin Sections: 2-3 µg/ml.
In paraffin embedded Human Skin, this antibody shows strong nuclear staining in selected cells of the basal layer. Data obtained from a previuos batch (different goat).
Background Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. As 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. 14-3-3 sigma may be required for Raf-1 kinase activity and phosphorylation, among many other functions.
Concentration 0.5 mg/ml
General Readings
  1. Lodygin D, Hermeking H. The role of epigenetic inactivation of 14-3-3sigma in human cancer. Cell Res. 2005 Apr;15(4):237-46. PubMed PMID: 15857578.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Buffer System:
Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
State:
Liquid purified Ig fraction.
Aff - Purified
Species Reactivity
Species reactivity (expected):
Mouse, Rat, Canine (Dog) and Porcine.
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody recognizes 4-3-3 sigma/Stratifin.

Accessory Products

Proteins and/or Positive Controls

Proteins for 14-3-3 protein sigma / SFN (11 products)

Catalog No. Species Pres. Purity   Source  

14-3-3 protein sigma / SFN

14-3-3 protein sigma / SFN Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

14-3-3 protein sigma / SFN

14-3-3 protein sigma / SFN Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €299.00
  OriGene Technologies, Inc.

14-3-3 protein sigma / SFN (1-248)

14-3-3 protein sigma / SFN Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €670.00
  OriGene Technologies GmbH

14-3-3 protein sigma / SFN (1-248)

14-3-3 protein sigma / SFN Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €250.00
  OriGene Technologies GmbH

14-3-3 protein sigma / SFN

14-3-3 protein sigma / SFN Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

14-3-3 protein sigma / SFN

14-3-3 protein sigma / SFN Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

14-3-3 protein sigma / SFN

14-3-3 protein sigma / SFN Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

14-3-3 protein sigma / SFN

14-3-3 protein sigma / SFN Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

14-3-3 protein sigma / SFN

14-3-3 protein sigma / SFN Human Purified 95 % E. coli
  GenWay Biotech Inc.

14-3-3 protein sigma / SFN

14-3-3 protein sigma / SFN Human Purified 95 % E. coli
  GenWay Biotech Inc.

14-3-3 protein sigma / SFN

Wetern Blot: 14-3-3 sigma/Stratifin Protein [NBP1-30172] - 15% SDS-PAGE (3ug)
  Novus Biologicals Inc.

Positive controls for 14-3-3 protein sigma / SFN (2 products)

Catalog No. Species Pres. Purity   Source  

14-3-3 sigma Lysate

Western Blot: 14-3-3 sigma/Stratifin  Lysate [NBL1-15882] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for SFN
  Novus Biologicals Inc.

SFN overexpression lysate

SFN overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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