AP11021PU-N 3xB Epitope Tag (CQYPALTQYPALTQYPALT) antibody

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Rabbit anti 3xB Epitope Tag (CQYPALTQYPALTQYPALT)

Product Description for 3xB Epitope Tag (CQYPALTQYPALTQYPALT)

Rabbit anti 3xB Epitope Tag (CQYPALTQYPALTQYPALT).
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for 3xB Epitope Tag (CQYPALTQYPALTQYPALT)

Product Category Primary Antibodies
Quantity 0.4 ml
Presentation Purified
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype Ig
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

KLH conjugated synthetic peptide encoding 3xBtag (CQYPALTQYPALTQYPALT) was used as antigen.
Application ELISA: 1/5,000.
Western Blot.
Background Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. BTag is an epitope tag composed of a 6 residue peptide, QYPALT, derived from the highly conserved region of the major core protein, VP7, of bluetongue (BT) viruses. This polyclonal antibody provides a simple solution to detect the expression of a B-tagged protein in cells.
Caution This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Concentration lot specific
General Readings
  1. Wang LF, Yu M, White JR, Eaton BT. BTag: a novel six-residue epitope tag for surveillance and purification of recombinant proteins. Gene. 1996 Feb 22;169(1):53-8. PubMed PMID: 8635749.
  2. du Plessis DH, Wang LF, Jordaan FA, Eaton BT. Fine mapping of a continuous epitope on VP7 of bluetongue virus using overlapping synthetic peptides and a random epitope library. Virology. 1994 Jan;198(1):346-9. PubMed PMID: 7505073.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Buffer System:
PBS containing 0.09% (W/V) Sodium Azide as preservative.
Liquid purified Ig fraction.
B Tag specific.

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