NSB921 40S ribosomal protein S6 (RPS6) antibody

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Rabbit anti Human 40S ribosomal protein S6 (RPS6)

Product Description for 40S ribosomal protein S6 (RPS6)

Rabbit anti Human 40S ribosomal protein S6 (RPS6).
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for 40S ribosomal protein S6 (RPS6)

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms 1
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human RPS6 that contains serine 236. The sequence is conserved in mouse and rat.
Add. information Research Areas: Cancer
Application Western Blot, This antibody is suitable for use in Western blotting. , Western Blot 1:1,000
Background 40S ribosomal protein S6 (also known as RPS6) is a ~31 kDa substrate of p70 S6 kinase (p70S6K) and a major component of translational machinery involved in protein synthesis, cell growth, proliferation, and metabolism. Phosphorylation of RPS6 is rapamycin and wortmannin-sensitive as its activation is mediated by mTOR and PI3K pathways. Ribosomal protein S6 undergoes phosphorylation on multiple serines in the carboxyl terminal region in the order 236,235,240,244,247, due to the positions of these amino acid residues on the a-helix. Hyperphosphorylation of ribosomal protein S6 stimulates protein synthesis that mediates progression through the cell cycle.
General Readings Pende, M., et al. (2004) S6K1(-/-)/S6K2(-/-) mice exhibit perinatal lethality and rapamycin-sensitive 5'-terminal oligopyrimidine mRNA translation and reveal a mitogen-activated protein kinase-dependent S6 kinase pathway. Mol. Cell. Biol. 24(8):3112-3124.
Mourani, P.M., et al. (2004) Unique, highly proliferative growth phenotype expressed by embryonic and neointimal smooth muscle cells is driven by constitutive Akt, mTOR, and p70S6K signaling and is actively repressed by PTEN. Circulation 109(10):1299-1306.
Lekmine, F., et al. (2004) Interferon-gamma engages the p70 S6 kinase to regulate phosphorylation of the 40S S6 ribosomal protein. Exp. Cell. Res. 295(1):173-182.
Tuhackova, Z., et al. (2004) IL2-dependent phosphorylation of 40S ribosomal protein S6 is controlled by PI-3K/mTOR signalling in CTLL2 cells. Int. J. Mol. Med. 13(4):601-605.
Shah, O.J., et al. (2003) Mitotic regulation of ribosomal S6 kinase 1 involves Ser/Thr, Pro phosphorylation of consensus and non-consensus sites by Cdc2. J. Biol. Chem. 278(18):16433-16442.
Stewart, M.J. and G. Thomas (1994) Mitogenesis and protein synthesis: a role for ribosomal protein S6 phosphorylation? Bioessays 16(11):809-815. Review.
Ferrari, S., et al. (1991) Mitogen-activated 70K S6 kinase. Identification of in vitro 40S ribosomal S6 phosphorylation sites. J. Biol. Chem. 266(33):22770-22775.
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Immunogen affinity purified
Aff - Purified
Ribosomal Protein S6 [Ser236]

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