AM33116PU-N 5-Hydroxymethylcytosine antibody

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0.1 mg / €380.00
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Mouse anti All Species 5-Hydroxymethylcytosine 4D9


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  • AM33116PU-N

Product Description for 5-Hydroxymethylcytosine

Mouse anti All Species 5-Hydroxymethylcytosine 4D9.
Presentation: Purified
Product is tested for MeDIP, Dot blot, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence.

Properties for 5-Hydroxymethylcytosine

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms 5' hydroxymethyl cytosine, 5'OH methyl cytosine, 5'OHmeC, 5-hmec
Presentation Purified
Reactivity All Species
Applications Dot, E, ICC/IF, MeDIP
Clonality Monoclonal
Clone 4D9
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Modified 5-Hydroxymethylcytosine found in DNA vertebrates.
Application ELISA: 1/1000 (See Figure.1)
Dot Blot: 1/2000 (See Table.1)
hMeDIP: 1-3 µg per Immunoprecipitaion.
Immunofluorescence: 1/500.
5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation.
5-hydroxymethylcytosine (5-hmC) is a modified base form of cytosine recently found in human/mouse brain and in embryonic stem cells. This DNA pyrimidine nitrogen base can be generated by oxidation of 5-methylcytosine, a reaction mediated by the ten-eleven translocation (TET) family of the 5-mC hydroxylases. The function of this base is still not elucidated but it is believed to play an important role in switching genes on and off.
Concentration 1.0 mg/ml
General Readings
  1. Li W, Liu M. Distribution of 5-hydroxymethylcytosine in different human tissues. J Nucleic Acids. 2011;2011:870726. doi: 10.4061/2011/870726. Epub 2011 Jun 9. PubMed PMID: 21772996. (Free PMC Article available, 3 images available)
  2. Guo JU, Su Y, Zhong C, Ming GL, Song H. Hydroxylation of 5-methylcytosine by TET1 promotes active DNA demethylation in the adult brain. Cell. 2011 Apr 29;145(3):423-34. doi: 10.1016/j.cell.2011.03.022. Epub 2011 Apr 14. PubMed PMID: 21496894. (Free PMC Article available, 7 images available)
Storage Upon receipt, store (in aliquots) at -20°C to -80°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography on Protein A
Buffer System:
10mM PBS, 0.15M Sodium Chloride, pH 7.4
0.01 % Thimerosal
Liquid purified IgG fraction
This antibody detects 5-Hydroxymethylcytosine (5-hmC). 
Expected to detect 5-Hydroxymethylcytosine in all Species.

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