Product Description for 5-Hydroxymethylcytosine
Mouse anti All Species 5-Hydroxymethylcytosine 4D9.
Presentation: Purified
Product is tested for MeDIP, Dot blot, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence.
Presentation: Purified
Product is tested for MeDIP, Dot blot, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence.
Properties for 5-Hydroxymethylcytosine
Product Category | Primary Antibodies |
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Quantity | 0.1 mg |
Synonyms | 5' hydroxymethyl cytosine, 5'OH methyl cytosine, 5'OHmeC, 5-hmec |
Presentation | Purified |
Reactivity | All Species |
Applications | Dot, E, ICC/IF, MeDIP |
Clonality | Monoclonal |
Clone | 4D9 |
Host | Mouse |
Isotype | IgG1 |
Shipping to | Worldwide |
PDF datasheet | View Datasheet |
Manufacturer | Acris Antibodies GmbH |
Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
Immunogen |
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Isotype control | AM03095PU-N |
Application | ELISA: 1/1000 (See Figure.1) Dot Blot: 1/2000 (See Table.1) hMeDIP: 1-3 µg per Immunoprecipitaion. Immunofluorescence: 1/500. |
Background | 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation. 5-hydroxymethylcytosine (5-hmC) is a modified base form of cytosine recently found in human/mouse brain and in embryonic stem cells. This DNA pyrimidine nitrogen base can be generated by oxidation of 5-methylcytosine, a reaction mediated by the ten-eleven translocation (TET) family of the 5-mC hydroxylases. The function of this base is still not elucidated but it is believed to play an important role in switching genes on and off. |
Concentration | 1.0 mg/ml |
General Readings |
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Storage | Upon receipt, store (in aliquots) at -20°C to -80°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
Format |
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Specificity |
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Accessory Products
Recommended Secondary Antibodies for 5-Hydroxymethylcytosine (11 products)
Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
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R1253AP |
Mouse IgG (H+L chain) antibody |
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Rabbit | AP | C, E, WB | ||||
Acris Antibodies GmbH | |||||||
R1253B |
Mouse IgG (H+L chain) antibody |
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Rabbit | Biotin | C, E, WB | ||||
Acris Antibodies GmbH | |||||||
R1253F |
Mouse IgG (H+L chain) antibody |
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Rabbit | FITC | E, ICC/IF | ||||
Acris Antibodies GmbH | |||||||
R1253HRP |
Mouse IgG (H+L chain) antibody |
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Rabbit | HRP | C, E, WB | ||||
Acris Antibodies GmbH | |||||||
R1253TR |
Mouse IgG (H+L chain) antibody |
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Rabbit | Texas Red | ICC/IF | ||||
Acris Antibodies GmbH | |||||||
R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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Rabbit | PE | F | ||||
Acris Antibodies GmbH | |||||||
R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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Goat | PE | |||||
Acris Antibodies GmbH | |||||||
R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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Donkey | PE | |||||
Acris Antibodies GmbH | |||||||
R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
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Goat | Cy2 | |||||
Acris Antibodies GmbH | |||||||
R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
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Goat | Cy3 | |||||
Acris Antibodies GmbH | |||||||
R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
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Goat | Cy5 | |||||
Acris Antibodies GmbH |