SM1872PS 5-Methyl Cytosine / 5-MeC antibody

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50 µg / €250.00
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Mouse anti Human, Mouse, Rat 5-Methyl Cytosine / 5-MeC 33D3

SM1872PS

Product Description for 5-Methyl Cytosine / 5-MeC

Mouse anti Human, Mouse, Rat 5-Methyl Cytosine / 5-MeC 33D3.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot, Flow Cytometry.

Properties for 5-Methyl Cytosine / 5-MeC

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms 5-MeCyd, 5-Methyl Cytidine, 5-Methylcytidine, 5MeC
Presentation Purified
Reactivity Hu, Ms, Rt
Applications C, F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone 33D3
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0Ag 14 myeloma cell line
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Flow Cytometry: Membrane permeabilisation may be required for this application. Cell pretreatment before staining is described in Ref.4 (Giraldo, A. M. et al.)
Immunoblotting.
Immunofluorescence.
Immunohistochemistry on Frozen and Paraffin Embedded Sections:
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
This antibody has been reported for use in Methylated DNA Immunoprecipitation (MeDIP).
Background Cytosine is a nucleobase whilst cytidine is a molecule (known as a nucleoside) that is formed when Cytosine is attached to a Ribose ring (also known as a Ribofuranose) via a beta-N1-glycosidic bond.
Concentration 1.0 mg/ml
General Readings
  1. Reynaud C, Bruno C, Boullanger P, Grange J, Barbesti S, Niveleau A. Monitoring of urinary excretion of modified nucleosides in cancer patients using a set of six monoclonal antibodies. Cancer Lett. 1992 Jan 31;61(3):255-62. PubMed PMID: 1739950.
  2. Habib, M. et al. (1999) DNA Global Hypomethylation in EBV - Transformed Interphase Nuclei. Exp. Cell. Res. 249: 46-53.
  3. Hernandez-Blazquez FJ, Habib M, Dumollard JM, Barthelemy C, Benchaib M, de Capoa A, et al. Evaluation of global DNA hypomethylation in human colon cancer tissues by immunohistochemistry and image analysis. Gut. 2000 Nov;47(5):689-93. PubMed PMID: 11034586. (Free PMC Article available, 3 images available)
  4. Giraldo AM, Lynn JW, Purpera MN, Godke RA, Bondioli KR. DNA methylation and histone acetylation patterns in cultured bovine fibroblasts for nuclear transfer. Mol Reprod Dev. 2007 Dec;74(12):1514-24. PubMed PMID: 17440941.
  5. Shen R, Tao L, Xu Y, Chang S, Van Brocklyn J, Gao JX. Reversibility of aberrant global DNA and estrogen receptor-alpha gene methylation distinguishes colorectal precancer from cancer. Int J Clin Exp Pathol. 2009;2(1):21-33. Epub 2008 Apr 20. PubMed PMID: 18830381. (Free PMC Article available, 6 images available)
  6. Pontes O, Lawrence RJ, Silva M, Preuss S, Costa-Nunes P, Earley K, et al. Postembryonic establishment of megabase-scale gene silencing in nucleolar dominance. PLoS One. 2007 Nov 7;2(11):e1157. PubMed PMID: 17987131. (Free PMC Article available, 5 images available)
  7. Yang F, Zhang L, Li J, Huang J, Wen R, Ma L, et al. Trichostatin A and 5-azacytidine both cause an increase in global histone H4 acetylation and a decrease in global DNA and H3K9 methylation during mitosis in maize. BMC Plant Biol. 2010 Aug 18;10:178. doi: 10.1186/1471-2229-10-178. PubMed PMID: 20718950. (Free PMC Article available, 7 images available)
  8. Suter JD, Howard DJ, Shi H, Caldwell CW, Fan X. Label-free DNA methylation analysis using opto-fluidic ring resonators. Biosens Bioelectron. 2010 Nov 15;26(3):1016-20. doi: 10.1016/j.bios.2010.08.050. Epub 2010 Aug 20. PubMed PMID: 20846848.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
10 mM PBS, pH 7.4
Preservatives:
0.01% Thimerosal
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human, Rat and Mouse.
Specificity
Specificity:
This antibody recognises the modified base 5-Methylcytidine (5-MeCyd) found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression.
Clone 33D3 has been developed to discriminate between the modified base 5-MeCyd and the normal counterpart Cytosine.
Reports suggest that in tumours, DNA is frequently globally hypomethylated compared to the DNA from normal tissue.

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