PP1005P2 6CKine / CCL21 antibody

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0.1 mg / €340.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human 6CKine / CCL21

PP1005P2

Product Description for 6CKine / CCL21

Rabbit anti Human 6CKine / CCL21.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Functional assay, Western blot / Immunoblot.

Properties for 6CKine / CCL21

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Beta chemokine Exodus-2, CCL-21, SCYA21, SLC, Secondary lymphoid-tissue chemokine, Small-inducible cytokine A21
Presentation Aff - Purified
Reactivity Hu
Applications E, FN, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
O00585
Immunogen:
Highly pure (>98%) 12.2 kDa E.coli derived recombinant Human Exodus-2.
GeneID:
6366
Add. information Centrifuge vial prior to opening!
Application Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human Exodus-2 (100.0 ng/ml), a concentration of 6.0-8.0 µg/ml of this antibody is required.
Indirect ELISA: To detect Human Exodus-2 by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Exodus-2.
Sandwich ELISA: To detect Human Exodus-2 by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Exodus-2 (PP1005B1 or PP1005B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Exodus-2.
Western Blot: To detect Human Exodus-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Exodus-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Background Exodus 2 is one of several CC cytokine genes clustered on the p arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterised by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).
General Readings
  1. Steiniger B, Rüttinger L, Barth PJ. The three-dimensional structure of human splenic white pulp compartments. J Histochem Cytochem. 2003 May;51(5):655-64. PubMed PMID: 12704213.
  2. Kriehuber E, Breiteneder-Geleff S, Groeger M, Soleiman A, Schoppmann SF, Stingl G, et al. Isolation and characterization of dermal lymphatic and blood endothelial cells reveal stable and functionally specialized cell lineages. J Exp Med. 2001 Sep 17;194(6):797-808. PubMed PMID: 11560995. (Free PMC Article available, 8 images available)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography.
Buffer System:
PBS, pH 7.2 without preservatives.
State:
Lyophilized purified Ig fraction.
Aff - Purified
Reconstitution:
Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody recognizes Human Exodus-2.
Gene ID 6366

Accessory Products

Proteins and/or Positive Controls

Proteins for 6CKine / CCL21 (13 products)

Catalog No. Species Pres. Purity   Source  

6CKine / CCL21

6CKine / CCL21 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

6CKine / CCL21

6CKine / CCL21 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
20 µg / €139.00
  OriGene Technologies, Inc.

6CKine / CCL21

6CKine / CCL21 Human > 98 %
Preparation: .
Purity Detail: >98%, as determined by Coomassie stained SDS-PAGE.
E. coli
10 µg / €105.00
  OriGene Technologies, Inc.

6CKine / CCL21 (24-134, His-tag)

Recombinant human CCL21, 24-134 aa, His-tagged: 15% SDS-PAGE (3 µg) Human Purified > 90 % by SDS-PAGE E. coli
0.25 mg / €1,000.00
  Acris Antibodies GmbH

6CKine / CCL21 (24-134, His-tag)

Recombinant human CCL21, 24-134 aa, His-tagged: 15% SDS-PAGE (3 µg) Human Purified > 90 % by SDS-PAGE E. coli
50 µg / €370.00
  Acris Antibodies GmbH

6CKine / CCL21

6CKine / CCL21 Human > 98 % pure by SDS-PAGE and HPLC analyses. E. coli
  Acris Antibodies GmbH

6CKine / CCL21

6CKine / CCL21 Human > 98 % pure by SDS-PAGE and HPLC analyses. E. coli
  Acris Antibodies GmbH

6CKine / CCL21

6CKine / CCL21 Human
  Abnova Taiwan Corp.

6CKine / CCL21

6CKine / CCL21 Human Purified
  Abnova Taiwan Corp.

6CKine / CCL21

6CKine / CCL21 Human Purified
  Abnova Taiwan Corp.

6CKine / CCL21

6CKine / CCL21 Human
  Abnova Taiwan Corp.

6CKine / CCL21

6CKine / CCL21 Human
  Abnova Taiwan Corp.

6CKine / CCL21

SDS-Page: CCL21 Protein [NBP1-37092] - CCL21, 14.5 kDa (132aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE
  Novus Biologicals Inc.

Positive controls for 6CKine / CCL21 (2 products)

Catalog No. Species Pres. Purity   Source  

CCL21 293T Cell Transient Overexpression Lysate(Denatured)

CCL21 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

CCL21 Lysate

Western Blot: CCL21 Lysate [NBL1-08851] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for CCL21
  Novus Biologicals Inc.
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