AM05288PU-N 6xHistidine Epitope Tag (HHHHHH) antibody

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0.1 mg / €370.00
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Quick Overview

Mouse anti 6xHistidine Epitope Tag IPA2C5.1

Product Description for 6xHistidine Epitope Tag

Mouse anti 6xHistidine Epitope Tag IPA2C5.1.
Properties: (HHHHHH)
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Applications WB
Clonality Monoclonal
Clone IPA2C5.1
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Hybridoma produced by the fusion of splenocytes from mice immunized with His-His-His-His-His-His synthetic peptide and mouse myeloma cells.
Isotype control AM03096PU-N
Application Western Blot.
Background 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.
Concentration Lot specific
General Readings
  1. Zentgraf H, Frey M, Schwinn S, Tessmer C, Willemann B, Samstag Y, et al. Detection of histidine-tagged fusion proteins by using a high-specific mouse monoclonal anti-histidine tag antibody. Nucleic Acids Res. 1995 Aug 25;23(16):3347-8. PubMed PMID: 7667114. (Free PMC Article available)
  2. Sisk WP, Bradley JD, Leipold RJ, Stoltzfus AM, Ponce de Leon M, Hilf M, et al. High-level expression and purification of secreted forms of herpes simplex virus type 1 glycoprotein gD synthesized by baculovirus-infected insect cells. J Virol. 1994 Feb;68(2):766-75. PubMed PMID: 8289380. (Free PMC Article available)
  3. Hosfield T, Lu Q. S. pombe expression vector with 6x(His) tag for protein purification and potential for ligation-independent cloning. Biotechniques. 1999 Jul;27(1):58-60. PubMed PMID: 10407663.
Storage Store the antibody at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Buffer System:
PBS containing 0.08% Sodium Azide as preservative.
Liquid (sterile filtered) purified IgG fraction.
This antibody is effective in detecting proteins expressed using the following vector systems, pET 28, 30 and 32 as well as Invitrogen's pIZ.
Specific for 6x-His tags only and for His tags no longer than 6 histidines.

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