AM10178PU-N 6xHistidine Epitope Tag (HHHHHH) antibody

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Mouse anti 6xHistidine Epitope Tag HIS.H8 / EH158


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  • AM10178PU-N
  • AM10178PU-N

Product Description for 6xHistidine Epitope Tag

Mouse anti 6xHistidine Epitope Tag HIS.H8 / EH158.
Properties: (HHHHHH)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Applications E, IP, WB
Clonality Monoclonal
Clone HIS.H8 / EH158
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

HHHHHH (6x His) synthetic peptide conjugated to KLH
Isotype control AM03110PU-N, SM12P
Application ELISA.
Western Blot:
Immunostaining: 1/500-1/2000. 
Background Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (6xHis) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni -chelating resins and purification by single step affinity chromatography. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site. The antibody is useful for detection, isolation and localization of the His-tag proteins.
Concentration 1.0 mg/ml

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Affinity Chromatography
Buffer System:
10mM PBS, pH 7.2
0.05% Sodium Azide
Liquid purified IgG fraction
Reacts with the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags).

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