AM11011SU-N 6xHistidine Epitope Tag (HHHHHH) antibody

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0.4 ml / €370.00
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Quick Overview

Mouse anti 6xHistidine Epitope Tag 6AT18

Product Description for 6xHistidine Epitope Tag

Mouse anti 6xHistidine Epitope Tag 6AT18.
Properties: (HHHHHH)
Presentation: Ascites
Product is tested for Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 0.4 ml
Presentation Ascites
Applications WB
Clonality Monoclonal
Clone 6AT18
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody.
Application ELISA: 1/5,000.
Western blotting: 1/1,000.
Background Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
Concentration 0.5 mg/mL
General Readings
  1. Unsal-Kacmaz, Keziban, et al. Mol. Cell. Biol. 2007 Apr 15;27(8):3131-3142. (Applications: WB)
  2. Partch, C.L., et al. Biochemistry 2005 Feb 11.44 (10): 3795-3805. (Applications: WB).
  3. Hochuli E, Döbeli H, Schacher A. New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues. J Chromatogr. 1987 Dec 18;411:177-84. PubMed PMID: 3443622.
  4. Yadav KK, Bar-Sagi D. Allosteric gating of Son of sevenless activity by the histone domain. Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3436-40. doi: 10.1073/pnas.0914315107. Epub 2010 Feb 4. PubMed PMID: 20133694. (Free PMC Article available, 4 images available)
  5. Mohammed Bahey-El-Din, et al. Expression of two Listeria monocytogenes antigens (P60 and LLO) in Lactococcus lactis and examination for use as live vaccine vectors J Med Microbiol. 2010 May 20
  6. Kemp MG, Akan Z, Yilmaz S, Grillo M, Smith-Roe SL, Kang TH, et al. Tipin-replication protein A interaction mediates Chk1 phosphorylation by ATR in response to genotoxic stress. J Biol Chem. 2010 May 28;285(22):16562-71. doi: 10.1074/jbc.M110.110304. Epub 2010 Mar 15. PubMed PMID: 20233725. (Free PMC Article available, 6 images available)
  7. Mongiat M, Marastoni S, Ligresti G, Lorenzon E, Schiappacassi M, Perris R, et al. The extracellular matrix glycoprotein elastin microfibril interface located protein 2: a dual role in the tumor microenvironment. Neoplasia. 2010 Apr;12(4):294-304. PubMed PMID: 20360940. (Free PMC Article available, 7 images available)
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS containing 0.09% (W/V) Sodium Azide as preservative.
State:
Ascites.
Ascites

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