discontinued

AM20706PU-N 6xHistidine Epitope Tag (HHHHHH) antibody

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0.1 mg / €300.00

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Mouse anti Mammalia 6xHistidine Epitope Tag HIS.H8

AM20706PU-N

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  • AM20706PU-N

Product Description for 6xHistidine Epitope Tag

Mouse anti Mammalia 6xHistidine Epitope Tag HIS.H8.
Properties: (HHHHHH)
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
Presentation Aff - Purified
Reactivity All., Mam
Applications ICC/IF, IP, WB
Clonality Monoclonal
Clone HIS.H8
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide (KLH-coupled) containing the His epitope 6x His (H-H-H-H-H-H).
Application Western blot: 1/1000-1/4000.
Immunoprecipitation: 1/200-1/400.
Immunostaining: 1/200-1/400.
Background Expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni++-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site.
Concentration 1.0 mg/ml
General Readings
  1. Terpe K. Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol. 2003 Jan;60(5):523-33. Epub 2002 Nov 7. PubMed PMID: 12536251.
  2. Xu L, Liu Y, He X. Expression and purification of soluble human programmed death-1 in Escherichia coli. Cell Mol Immunol. 2006 Apr;3(2):139-43. PubMed PMID: 16696901.
  3. Els Conrath K, Lauwereys M, Wyns L, Muyldermans S. Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs. J Biol Chem. 2001 Mar 9;276(10):7346-50. Epub 2000 Oct 25. PubMed PMID: 11053416.
  4. Maniattis, T., et al. 1982. Molecular Cloning. Cold Spring Laboratory, Cold Spring Harbor, NY.
  5. Hochuli E. Large-scale chromatography of recombinant proteins. J Chromatogr. 1988 Jul 1;444:293-302. PubMed PMID: 3060478.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography
Buffer System:
10mM PBS, pH 7.2
Preservatives:
0.05% Sodium Azide
State:
Liquid purified IgG fraction
Aff - Purified
Specificity
Specificity:
His-tag monoclonal antibody recognizes His-tagged tagged proteins overexpressed in cells, including both amino-or carboxy-termini of targeted proteins in transfected mammalian cells.
Reacts with Human, Mouse, Rat and Others.

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