AM26391PU-N 6xHistidine Epitope Tag (HHHHHH) antibody

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0.1 mg / €310.00
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Quick Overview

Mouse anti 6xHistidine Epitope Tag 10A12

Product Description for 6xHistidine Epitope Tag

Mouse anti 6xHistidine Epitope Tag 10A12.
Properties: (HHHHHH)
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Applications WB
Clonality Monoclonal
Clone 10A12
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Synthetic 6 x Histidine peptide conjugated with KLH
Isotype control AM03095PU-N
Application Western blot.
Background Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (6xHis) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni -chelating resins and purification by single step affinity chromatography. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site. The antibody is useful for detection, isolation and localization of the His-tag proteins.
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
0.01M PBS pH 7.2
Endotoxin level:
Restore with appropriate volume of dsitilled water to reach a final concentration of 1.0 mg/ml.
Lyophilized purified IgG fraction
This antibody binds to His-tagged recombinant protein, but not with KLH.

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