AM33047PU-S 6xHistidine Epitope Tag (HHHHHH) antibody

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25 µg / €230.00
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Mouse anti All Species 6xHistidine Epitope Tag J099B12

AM33047PU-S

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  • AM33047PU-S

Product Description for 6xHistidine Epitope Tag

Mouse anti All Species 6xHistidine Epitope Tag J099B12.
Properties: (HHHHHH)
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Flow Cytometry, Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 25 µg
Synonyms 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
Presentation Purified
Reactivity All Species
Applications F, ICC/IF, WB
Clonality Monoclonal
Clone J099B12
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
KLH-conjugated His-tag peptide.
Isotype control AM03095PU-N
Application Western blotting: Each lot of this antibody is quality control tested. 
Use 0.1-0.2 µg/ml antibody dilution buffer for each mini-gel.
Immunofluorescence: Use 0.5-1.0 µg/ml antibody dilution buffer. 
Flow Cytometric analysis of intracellularly-stained cells.
Background Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (6xHis) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni -chelating resins and purification by single step affinity chromatography. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site.
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Burritt JB, Quinn MT, Jutila MA, Bond CW, Jesaitis AJ. Topological mapping of neutrophil cytochrome b epitopes with phage-display libraries. J Biol Chem. 1995 Jul 14;270(28):16974-80. PubMed PMID: 7622517.
  2. Taylor RM, Jesaitis AJ. Immunoaffinity purification of human phagocyte flavocytochrome b and analysis of conformational dynamics. Methods Mol Biol. 2007;412:429-37. doi: 10.1007/978-1-59745-467-4_26. PubMed PMID: 18453126.
General Readings
  1. Tymms MJ, Ng AY, Thomas RS, Schutte BC, Zhou J, Eyre HJ, et al. A novel epithelial-expressed ETS gene, ELF3: human and murine cDNA sequences, murine genomic organization, human mapping to 1q32.2 and expression in tissues and cancer. Oncogene. 1997 Nov 13;15(20):2449-62. PubMed PMID: 9395241.
  2. Hou CL, Tang Cj, Roffler SR, Tang TK. Protein 4.1R binding to eIF3-p44 suggests an interaction between the cytoskeletal network and the translation apparatus. Blood. 2000 Jul 15;96(2):747-53. PubMed PMID: 10887144.
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography
Buffer System:
PBS, pH 7.2
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
This J099B12 Monoclonal antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats.
It can be used for Western blotting to detect linear His repeat (His6 or more). It can also be used for Intracellularly Flow Cytometry and Immunofluorescence staining of His-Tagged protein.

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