AP23426PU-N 6xHistidine Epitope Tag (HHHHHH) antibody

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Rabbit anti Mammalia 6xHistidine Epitope Tag

Product Description for 6xHistidine Epitope Tag

Rabbit anti Mammalia 6xHistidine Epitope Tag.
Properties: (HHHHHH)
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
Presentation Aff - Purified
Reactivity All., Mam
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Synthetic peptide containing the His epitope 6x His (H-H-H-H-H-H).
Application Western Blot (1/500-1/1000).
Background Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (6xHis) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni -chelating resins and purification by single step affinity chromatography. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site. The antibody is useful for detection, isolation and localization of the His-tag proteins.
Concentration 1.0 mg/ml
General Readings
  1. Terpe K. et al. (2003) Appl Microbiol Biotechnol; 60(5):523-533.
  2. Xu L. et al. (2006) Cell Mol Immunol; 3(2):139-143.
  3. Els Conrath, K., et al. ( 2001) J. Biol. Chem. 276: 7346-7350.
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Immunoaffinity Chromatography using epitope-specific peptide.
Buffer System:
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
Liquid purified Ig fraction.
Aff - Purified
His-Tag Polyclonal antibody recognizes His-tagged proteins overexpressed in cells, including both amino-or carboxy-termini of targeted proteins in transfected mammalian cells.

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