AP31792PU-N 6xHistidine Epitope Tag (HHHHHH) antibody

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0.4 ml / €380.00
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Quick Overview

Chicken anti 6xHistidine Epitope Tag

Product Description for 6xHistidine Epitope Tag

Chicken anti 6xHistidine Epitope Tag.
Properties: (HHHHHH)
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 0.4 ml
Synonyms 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
Presentation Purified
Applications WB
Clonality Polyclonal
Host Chicken
Isotype Ig
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
6X-Histidine peptide conjugated to Keyhole Limpet Hemocyanin protein and emulsified in Freund’s adjuvants.
After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using 6X-Histidine peptide conjugated to an agarose matrix.
To make up the final mixture, the IgY fraction was spiked with affinity purified antibody, mixed with Glycerol and Thimerosal, and filter-sterilized.
Application Western Blot.
Recommended Dilutions: Use 1/5000-1/10000 as starting points for western blotting.
Background In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called “tags” can be used e.g. to determine the cellular localization or to quantify proteins. The polyhistidine “tag” (His-tag) is the most used affinity epitope for the purification of recombinant proteins. Proteins with a polyhistidine tag (e.g. 6xHis or 8xHis) can be purified in one step using a metal-chelate column (e.g. Ni2+, Zn2+, Cu2+ or Co2+) and imidazole as eluent. This method now is a very attractive system for the purification of larger amounts proteins for structural and functional studies. So far His-tagged proteins were successfully purified from different expression systems like E. coli, yeast, insect cells and plant cells. An important requirement beside the efficient and robust purification method is the availability of a fast detection system for checking the purification steps of these His-tagged proteins if no specific antibody is available.
Concentration 10 mg/ml (based on absorbance at 280 nm)
Storage This antibody recognises the 6xHIS-Tag with high titre and high specificity in Immuoprecipitation.
Quality Control: This antibody preparation was analyzed by Western blotting (at a dilution of 1/10000) using tissue extracts expressing a recombinant protein tagged with the 6X-HIS marker. Secondary antibodies were HRP-labeled affinity-purified Goat anti-Chicken IgY (1/5000 dilution).
Format
Buffer System:
Sodium Phosphate-Buffered (10 mM) isotonic (0.9%, w/v) Saline (PBS, pH 7.2) (50% by volume) with 50% Glycerol as an anti-freezing agent and 0.01% Thimerosal as preservative.
State:
Mixture of IgY and Affinity-purified fractions
Purified
Specificity
Specificity:
This antibody recognizes the 6xHIS-Tag with high titre and high specificity in Western Blot.
Quality Control: This antibody preparation was analyzed by Western blotting (at a dilution of 1/10000) using tissue extracts expressing a recombinant protein tagged with the 6X-HIS marker. Secondary antibodies were HRP-labeled affinity-purified Goat anti-Chicken IgY (1/5000 dilution).

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