AS11 1771 6xHistidine Epitope Tag (HHHHHH) antibody

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Mouse anti 6xHistidine Epitope Tag HIS.H8 / EH158

AS11 1771

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  • AS11 1771

Product Description for 6xHistidine Epitope Tag

Mouse anti 6xHistidine Epitope Tag HIS.H8 / EH158.
Properties: (HHHHHH)
Presentation: Purified
Product is tested for Immunoprecipitation, Enzyme Immunoassay, Dot blot, Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 50 µg
Presentation Purified
Applications Dot, E, IP, WB
Clonality Monoclonal
Clone HIS.H8 / EH158
Host Mouse
Isotype IgG2b
Shipping to Europe only
PDF datasheet View Datasheet
Manufacturer AgriSera AB

Datasheet Extract

KLH-conjugated synthetic peptide 6xHis
Isotype control AM03110PU-N, SM12P
Application western blot (WB), dot blot (Dot), ELISA (ELISA), immunoprecipiation (IP), immunlocalization (IL). 
recommended dilution 1 : 1000 with standard ECL (WB) 
working dilution for ELISA, IL and IP needs to be determined experimentally.
Background His-Tag is a polyhistidine tag which consists of 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies.
Storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid
repeated freeze-thaw cycles. For storage at 4°C for several days to weeks,
sodium azide to final 0.1 % can be added. Please, remember to spin tubes briefly
prior to opening them to avoid any losses that might occur from lyophilized
material adhering to the cap or sides of the tubes.
Buffer System:
10 mM PBS, pH 7.2
mouse monoclonal IgG2b, clone HIS.H8 / EH158 lyophilized
50 μg for reconstitution add 50 μl, of sterile water.

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