DP3514 6xHistidine Epitope Tag (HHHHHH) antibody

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50 µg / €210.00
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Rabbit anti 6xHistidine Epitope Tag

DP3514

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  • DP3514

Product Description for 6xHistidine Epitope Tag

Rabbit anti 6xHistidine Epitope Tag.
Properties: (HHHHHH)
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 50 µg
Presentation Aff - Purified
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Different highly purified 6x His-tagged proteins (C-terminal) produced in insect cells.
Application Western blot (0.5-1 µg/ml): The antibody was tested using several His-tagged proteins expressed in insect cells and E. coli.
Background In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called “tags” can be used e.g. to determine the cellular localization or to quantify proteins. The polyhistidine “tag” (His-tag) is the most used affinity epitope for the purification of recombinant proteins [1]. Proteins with a polyhistidine tag (e.g. 6xHis or 8xHis) can be purified in one step using a metal-chelate column (e.g. Ni2+, Zn2+, Cu2+ or Co2+) and imidazole as eluent. This method now is a very attractive system for the purification of larger amounts proteins for structural and functional studies. So far His-tagged proteins were successfully purified from different expression systems like E. coli, yeast, insect cells and plant cells [1,2]. An important requirement beside the efficient and robust purification method is the availability of a fast detection system for checking the purification steps of these His-tagged proteins if no specific antibody is available.
General Readings
  1. Terpe K, Appl. Micrbiol. Biotechnol. 60:523, 2003.
  2. Leelavathi et al, Molecular Breeding 11:49, 2003.
  3. Bernhardt et al, Laborwelt 5:18, 2004 (German).
Storage The lyophilized IgG is stable at 2-8°C for one month from despatch and for one year when kept at -20°C.
The reconstituted antibody can be stored at 2-8°C for one month or at -20°C for one year.
Avoid repeated freezing and thawing.
Format
Purification:
Antigen Affinity Chromatography using a His-peptide as matrix.
Buffer System:
PBS, pH 7.4, without preservatives.
State:
Lyophilized purified IgG fraction.
Aff - Purified
Reconstitution:
Restore in sterile water to a concentration of > 0.5 mg/ml.
Specificity
Specificity:
Western analysis with several His-tagged proteins either expressed in insect cells or E.coli showed that the antibody recognizes all tested proteins fused to a C-terminal but not to a N-terminal His-tag.
The antibody might be a very good tool to test supernatants or cell lysates for expression of recombinant proteins.
The anti-His-Tag antibody is able to detect recombinant proteins in the conditioned media from insect cells and total lysate from E.coli.

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