DP3514 6xHistidine Epitope Tag (HHHHHH) antibody

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50 µg / €210.00
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Rabbit anti 6xHistidine Epitope Tag


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  • DP3514

Product Description for 6xHistidine Epitope Tag

Rabbit anti 6xHistidine Epitope Tag.
Properties: (HHHHHH)
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 50 µg
Presentation Aff - Purified
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Different highly purified 6x His-tagged proteins (C-terminal) produced in insect cells.
Application Western blot (0.5-1 µg/ml): The antibody was tested using several His-tagged proteins expressed in insect cells and E. coli.
Background In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called “tags” can be used e.g. to determine the cellular localization or to quantify proteins. The polyhistidine “tag” (His-tag) is the most used affinity epitope for the purification of recombinant proteins [1]. Proteins with a polyhistidine tag (e.g. 6xHis or 8xHis) can be purified in one step using a metal-chelate column (e.g. Ni2+, Zn2+, Cu2+ or Co2+) and imidazole as eluent. This method now is a very attractive system for the purification of larger amounts proteins for structural and functional studies. So far His-tagged proteins were successfully purified from different expression systems like E. coli, yeast, insect cells and plant cells [1,2]. An important requirement beside the efficient and robust purification method is the availability of a fast detection system for checking the purification steps of these His-tagged proteins if no specific antibody is available.
General Readings
  1. Terpe K, Appl. Micrbiol. Biotechnol. 60:523, 2003.
  2. Leelavathi et al, Molecular Breeding 11:49, 2003.
  3. Bernhardt et al, Laborwelt 5:18, 2004 (German).
Storage The lyophilized IgG is stable at 2-8°C for one month from despatch and for one year when kept at -20°C.
The reconstituted antibody can be stored at 2-8°C for one month or at -20°C for one year.
Avoid repeated freezing and thawing.
Antigen Affinity Chromatography using a His-peptide as matrix.
Buffer System:
PBS, pH 7.4, without preservatives.
Lyophilized purified IgG fraction.
Aff - Purified
Restore in sterile water to a concentration of > 0.5 mg/ml.
Western analysis with several His-tagged proteins either expressed in insect cells or E.coli showed that the antibody recognizes all tested proteins fused to a C-terminal but not to a N-terminal His-tag.
The antibody might be a very good tool to test supernatants or cell lysates for expression of recombinant proteins.
The anti-His-Tag antibody is able to detect recombinant proteins in the conditioned media from insect cells and total lysate from E.coli.

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