Product Description for 6xHistidine Epitope Tag
Rabbit anti 6xHistidine Epitope Tag.
Properties: (HHHHHH)
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.
Properties: (HHHHHH)
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.
Properties for 6xHistidine Epitope Tag
Product Category | Primary Antibodies |
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Quantity | 50 µg |
Presentation | Aff - Purified |
Applications | WB |
Clonality | Polyclonal |
Host | Rabbit |
Shipping to | Worldwide |
PDF datasheet | View Datasheet |
Manufacturer | Acris Antibodies GmbH |
Material safety datasheet | MSDS for Polyclonal Antibodies (de) |
Datasheet Extract
Immunogen |
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Application | Western blot (0.5-1 µg/ml): The antibody was tested using several His-tagged proteins expressed in insect cells and E. coli. |
Background | In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called “tags” can be used e.g. to determine the cellular localization or to quantify proteins. The polyhistidine “tag” (His-tag) is the most used affinity epitope for the purification of recombinant proteins [1]. Proteins with a polyhistidine tag (e.g. 6xHis or 8xHis) can be purified in one step using a metal-chelate column (e.g. Ni2+, Zn2+, Cu2+ or Co2+) and imidazole as eluent. This method now is a very attractive system for the purification of larger amounts proteins for structural and functional studies. So far His-tagged proteins were successfully purified from different expression systems like E. coli, yeast, insect cells and plant cells [1,2]. An important requirement beside the efficient and robust purification method is the availability of a fast detection system for checking the purification steps of these His-tagged proteins if no specific antibody is available. |
General Readings |
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Storage | The lyophilized IgG is stable at 2-8°C for one month from despatch and for one year when kept at -20°C. The reconstituted antibody can be stored at 2-8°C for one month or at -20°C for one year. Avoid repeated freezing and thawing. |
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Specificity |
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Accessory Products
Recommended Secondary Antibodies for 6xHistidine Epitope Tag (9 products)
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R1364AP |
Rabbit IgG (H+L chain) antibody |
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Goat | AP | Rb | E, WB | |||
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R1364B |
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Goat | Biotin | Rb | E, WB | |||
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R1364F |
Rabbit IgG (H+L chain) antibody |
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Goat | FITC | Rb | E, ICC/IF, WB | |||
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R1364HRP |
Rabbit IgG (H+L chain) antibody |
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Goat | HRP | Rb | E, P, WB | |||
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R1364T |
Rabbit IgG (H+L chain) antibody |
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Goat | TRITC | Rb | E, F, ICC/IF | |||
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R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
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Donkey | PE | Rb | F | |||
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R1458C2 |
Rabbit IgG (H+L chain, adsorbed) antibody |
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Goat | Cy2 | |||||
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R1458C3 |
Rabbit IgG (H+L chain, adsorbed) antibody |
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Goat | Cy3 | |||||
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R1458C5 |
Rabbit IgG (H+L chain, adsorbed) antibody |
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Goat | Cy5 | |||||
Acris Antibodies GmbH |