NB100-63173 6xHistidine Epitope Tag (HHHHHH) antibody

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0.1 ml / €380.00
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Mouse anti 6xHistidine Epitope Tag AD1.1.10

NB100-63173

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  • NB100-63173
  • NB100-63173

Product Description for 6xHistidine Epitope Tag

Mouse anti 6xHistidine Epitope Tag AD1.1.10.
Properties: (HHHHHH)
Presentation: HRP
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation HRP
Applications E, WB
Clonality Monoclonal
Clone AD1.1.10
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
PAX6 transcription factor linked to histidine tag
Application ELISA, Western Blot, This antibody is useful for ELISA and Western Blot. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. It reacts against all His tagged proteins so far tested. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. This product is routinely tested in ELISA and Western blotting on histidine tagged ubiquitin., ELISA Western Blot
Background The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Concentration 1 mg/ml
General Readings
  1. Els Conrath K, Lauwereys M, Wyns L, Muyldermans S. Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs. J Biol Chem. 2001 Mar 9;276(10):7346-50. Epub 2000 Oct 25. PubMed PMID: 11053416.
  2. Bahi A, Boyer F, Kafri T, Dreyer JL. CD81-induced behavioural changes during chronic cocaine administration: in vivo gene delivery with regulatable lentivirus. Eur J Neurosci. 2004 Mar;19(6):1621-33. PubMed PMID: 15066158.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Label:
HRP
Purification:
protein G purified
Buffer System:
0.01% Thimerosal
Purity:
protein G purified
Specificity
Specificity:
This antibody recognizes proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags.
Species:
Cross reacts with synthetic peptide.

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