NB100-64150 6xHistidine Epitope Tag (HHHHHH) antibody

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0.1 ml / €360.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti 6xHistidine Epitope Tag AD1.1.10

Product Description for 6xHistidine Epitope Tag

Mouse anti 6xHistidine Epitope Tag AD1.1.10.
Properties: (HHHHHH)
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation FITC
Applications F
Clonality Monoclonal
Clone AD1.1.10
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
PAX6 transcription factor linked to histidine tag
Application flow cytometry / FACS analysis, This antibody is useful for flow cytometry., flow cytometry / FACS analysis 1:10
Background The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Concentration 0.1 mg/ml
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Label:
FITC
Purification:
protein G purified
Buffer System:
0.09% Sodium Azide
Purity:
protein G purified
Specificity
Specificity:
This antibody recognizes proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. It reacts against all His tagged proteins so far tested. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.

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