NB100-64768 6xHistidine Epitope Tag (HHHHHH) antibody

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0.1 mg / €330.00
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Mouse anti All Species 6xHistidine Epitope Tag AD1.1.10

Product Description for 6xHistidine Epitope Tag

Mouse anti All Species 6xHistidine Epitope Tag AD1.1.10.
Properties: (HHHHHH)
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity All Species
Applications C, E, IP
Clonality Monoclonal
Clone AD1.1.10
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
PAX6 transcription factor linked to histidine tag
Isotype control AM03095PU-N
Application ELISA, Immunohistochemistry-Frozen, immunoprecipitation, This antibody is useful for Immunohistochemistry-Frozen, ELISA, immunoprecipitation, Immunohistochemistry-Frozen ELISA immunoprecipitation
Background The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Concentration 1 mg/ml
General Readings
  1. Els Conrath K, Lauwereys M, Wyns L, Muyldermans S. Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs. J Biol Chem. 2001 Mar 9;276(10):7346-50. Epub 2000 Oct 25. PubMed PMID: 11053416.
  2. Bahi A, Boyer F, Kafri T, Dreyer JL. CD81-induced behavioural changes during chronic cocaine administration: in vivo gene delivery with regulatable lentivirus. Eur J Neurosci. 2004 Mar;19(6):1621-33. PubMed PMID: 15066158.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Purification:
protein G purified
Buffer System:
0.09% Sodium Azide
Purity:
protein G purified
State:
Purified
Specificity
Specificity:
recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. It reacts against all His tagged proteins so far tested. In Western blotting of bacterial extracts clone ADI.I.10 has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
Species:
All Species

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