NBP1-18582 6xHistidine Epitope Tag (HHHHHH) antibody

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Rabbit anti 6xHistidine Epitope Tag


Product Description for 6xHistidine Epitope Tag

Rabbit anti 6xHistidine Epitope Tag.
Properties: (HHHHHH)
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 0.05 mg
Synonyms 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
Presentation Aff - Purified
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Application Western Blot, This is useful for the following applications: Western Blot: The antibody was tested using several His-tagged proteins expressed in insect cells and E. coli. The antibody detect only proteins with a C-terminal His-tag! The anti-His-tag is able to detect recombinant proteins in the conditioned media from insect cells and total lysate from E. coli. We recommend to use the IgG at a concentration of 0.5 , Western Blot 0.5-1 ug/ml,
Background The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
No Preservative
Immunogen affinity purified
Aff - Purified

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