R1181 6xHistidine Epitope Tag (HHHHHH) antibody

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0.1 mg / €310.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti 6xHistidine Epitope Tag

Product Description for 6xHistidine Epitope Tag

Rabbit anti 6xHistidine Epitope Tag.
Properties: (HHHHHH)
Presentation: Aff - Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Aff - Purified
Applications C, E, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide
Application Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins.
This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for Immunoprecipitation and Immunocytochemistry.
Recommended Dilutions:
ELISA: 1/10,000-1/40,000.
Western blot: 1/500-1/1,000.
Immunohistochemistry: 1/500-1/2,000.
Background Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Yi CE, Ba L, Zhang L, Ho DD, Chen Z. Single amino acid substitutions in the severe acute respiratory syndrome coronavirus spike glycoprotein determine viral entry and immunogenicity of a major neutralizing domain. J Virol. 2005 Sep;79(18):11638-46. PubMed PMID: 16140741. (Free PMC Article available, 7 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
Liquid (sterile filtered) purified Ig fraction.
Aff - Purified
This polyclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested.
In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
The antibody recognizes the His tag fused either to the amino- or carboxy-termini of targeted proteins.

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