discontinued

R1181D8 6xHistidine Epitope Tag (HHHHHH) antibody

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0.1 mg / €350.00

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Rabbit anti 6xHistidine Epitope Tag

Product Description for 6xHistidine Epitope Tag

Rabbit anti 6xHistidine Epitope Tag.
Properties: (HHHHHH)
Presentation: IRDYE800
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
Presentation IRDYE800
Applications ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide
Application LI-COR Odyssey(R) BLOT: 1/5,000 - 1/25,000.
LI-COR In-Cell Western(R).
Background Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged proteins biochemical properties. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings Bayer & Wilchek, Methods in Enzymology, 184; 138-160, 1990.
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
IRDye® 800 (MW 1166.2)
IRDYE800
Absorption emission:
774 nm / 800 nm
Molar radio:
2,7 moles IRDye® 800 per mole of Rabbit IgG
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA and 0.01% (w/v) Sodium Azide
State:
Lyophilized purified Ig
Reconstitution:
Restore with 0.1 ml of deionized water (or equivalent).
Specificity
Specificity:
This antibody is directed against the 6X His motif.

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