discontinued

R1181DL6 6xHistidine Epitope Tag (HHHHHH) antibody

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0.1 mg / €400.00

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Rabbit anti 6xHistidine Epitope Tag

R1181DL6

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  • R1181DL6
  • R1181DL6

Product Description for 6xHistidine Epitope Tag

Rabbit anti 6xHistidine Epitope Tag.
Properties: (HHHHHH)
Presentation: DyLight549
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
Presentation DyLight549
Applications E, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide corresponding to the 6X HIS epitope tag (H-H-H-H-H-H) conjugated to KLH using maleimide
Application Western Blot: >1/20,000.
ELISA >1/10,000.
Immunofluorescence: >1/5,000.
Background Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged proteins biochemical properties. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings Bayer & Wilchek, Methods in Enzymology, 184; 138-160, 1990.
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
DyLight(TM) 549 (MW 981.85)
DyLight549
Absorption emission:
550 nm / 568 nm
Molar radio:
3,6 moles DyLight(TM) 549 per mole of Rabbit IgG
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA and 0.01% (w/v) Sodium Azide
State:
Lyophilized purified Ig
Reconstitution:
Restore with 0.1 ml of deionized water (or equivalent).
Specificity
Specificity:
This antibody is directed against the 6X His motif.

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