discontinued

R1181DL7 6xHistidine Epitope Tag (HHHHHH) antibody

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0.1 mg / €400.00

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Rabbit anti 6xHistidine Epitope Tag

R1181DL7

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  • R1181DL7
  • R1181DL7

Product Description for 6xHistidine Epitope Tag

Rabbit anti 6xHistidine Epitope Tag.
Properties: (HHHHHH)
Presentation: DyLight649
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
Presentation DyLight649
Applications E, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Add. information The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Application Suitable for monitoring expression of His-tagged fusion proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for Immunoprecipitation and Immunocytochemistry.
Recommended Dilutions:
Western Blot: > 1/20,000.
ELISA: >1 /10,000.
Immunofluorescence: > 1/5,000.
Background Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged proteins biochemical properties. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Bayer & Wilchek, Methods in Enzymology, 184; 138-160, 1990 (Conjugation).
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
DyLight(TM) 649 (MW 1,008.02)
DyLight649
Absorption emission:
646 nm / 674 nm
Molar radio:
1.8 moles DyLight(TM) 649 per mole of Rabbit IgG
Purification:
Affinity Chromatography.
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
State:
Lyophilized purified Ig fraction.
Reconstitution:
Restore with 0.1 ml of deionized water (or equivalent).
Specificity
Specificity:
This ployclonal anti-6X His tag antibody is directed against the 6X HIS epitope tag and is useful in determining its presence in over expressed proteins in various assays.
The antibody recognizes the 6X HIS epitope tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

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