SM1693F 6xHistidine Epitope Tag (HHHHHH) antibody

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0.1 mg / €430.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti 6xHistidine Epitope Tag AD1.1.10

Product Description for 6xHistidine Epitope Tag

Mouse anti 6xHistidine Epitope Tag AD1.1.10.
Properties: (HHHHHH)
Presentation: FITC
Product is tested for Immunocytochemistry/Immunofluorescence, Flow Cytometry.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation FITC
Applications F, ICC/IF
Clonality Monoclonal
Clone AD1.1.10
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

PAX6 transcription factor linked to histidine tag.
Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Application Immunofluorescence.
Flow Cytometry: Use 10 µl of 1/10 diluted antibody to label 1x106 cells in 100 µl.
Background 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.
Concentration 0.1 mg/ml
General Readings
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Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Fluorescein Isothiocyanate Isomer 1
Affinity Chromatography on Protein G
Buffer System:
PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer
Liquid purified IgG fraction
This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. It reacts against all His tagged proteins so far tested.
In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. 
This antibody is routinely tested in Western blotting on Histidine tagged recombinant proteins and reacts against all Histidine-tagged proteins so far tested.

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