SM1693HRP 6xHistidine Epitope Tag (HHHHHH) antibody

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0.1 mg / €580.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti 6xHistidine Epitope Tag AD1.1.10

Product Description for 6xHistidine Epitope Tag

Mouse anti 6xHistidine Epitope Tag AD1.1.10.
Properties: (HHHHHH)
Presentation: HRP
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation HRP
Applications E, WB
Clonality Monoclonal
Clone AD1.1.10
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

PAX6 transcription factor linked to histidine tag.
Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Application This product is routinely tested in ELISA and Western blotting on Histidine tagged ubiquitin.
ELISA: 1/20-1/1000.
Western Blot: 1/1000-1/5000.
Background 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.
Concentration 1.0 mg/ml
General Readings
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Storage Store the antibody undiluted at 2-8°C.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.
Horseradish Peroxidase
Affinity Chromatography on Protein G
Buffer System:
PBS, pH 7.4 containing 0.01% Thiomersal as preservative and a HRP stabilizer
Liquid purified IgG fraction
This antibody recognizes proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. It reacts against all His tagged proteins so far tested. 
Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian (Hoffmann et al. 2007) and Hwang et al. 2008) and non-mammalian (Zheng et al. 2007; Gunnarsen et al. 2010; and de Vooght et al. 2012) cell lines.
In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.

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