SM1693R 6xHistidine Epitope Tag (HHHHHH) antibody

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1 ml / €390.00

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Mouse anti 6xHistidine Epitope Tag AD1.1.10


Product Description for 6xHistidine Epitope Tag

Mouse anti 6xHistidine Epitope Tag AD1.1.10.
Properties: (HHHHHH)
Presentation: PE
Product is tested for Flow Cytometry.

Properties for 6xHistidine Epitope Tag

Product Category Primary Antibodies
Quantity 1 ml
Synonyms 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
Presentation PE
Applications F
Clonality Monoclonal
Clone AD1.1.10
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

PAX6 transcription factor linked to histidine tag.
Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Application Flow Cytometry: Use 10 ul of Neat-1/5 diluted antibody to label 1x10e6 cells in 100 ul.
Concentration 0.1 mg/ml
General Readings
  1. Els Conrath K, Lauwereys M, Wyns L, Muyldermans S. Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs. J Biol Chem. 2001 Mar 9;276(10):7346-50. Epub 2000 Oct 25. PubMed PMID: 11053416.
  2. Bahi A, Boyer F, Kafri T, Dreyer JL. CD81-induced behavioural changes during chronic cocaine administration: in vivo gene delivery with regulatable lentivirus. Eur J Neurosci. 2004 Mar;19(6):1621-33. PubMed PMID: 15066158.
Storage Store the antibody undiluted at 2-8°C.
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
R. Phycoerythrin (RPE)
Affinity Chromatography on Protein G
Buffer System:
PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
Lyophilized purified IgG fraction.
Restore with 1.0 ml distilled water
This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags.
The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. It reacts against all His tagged proteins so far tested. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.

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