NB400-105 ABCA1 antibody

See related secondary antibodies

Search for all "ABCA1"

0.1 ml / €330.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Canine, Chicken, Hamster, Human, Mouse, Porcine, Rat ABCA1

NB400-105

More Views

  • NB400-105
  • NB400-105
  • NB400-105
  • NB400-105

Product Description for ABCA1

Rabbit anti Canine, Chicken, Hamster, Human, Mouse, Porcine, Rat ABCA1.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, ChIP assay, Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for ABCA1

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms ABC-1, ABC1, ATP-binding cassette sub-family A member 1, CERP
Presentation Aff - Purified
Reactivity Can, Chk, Hst, Hu, Ms, Por, Rt
Applications C, ChIP, E, F, ICC/IF, IP, P, WB
Clonality Polyclonal
Host Rabbit
Molecular weight 220 kDa
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
O95477
Immunogen:
Partial peptide sequence (peptide used resides somewhere between a.a 1100-1300) of the human ABCA1 gene. Actual immunogen sequence is proprietary information.
GeneID:
19
Application Western Blot, Chromatin Immunoprecipitation, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation.

Dilutions:
 
  • Western Blot 1:500
  • Chromatin Immunoprecipitation
  • ELISA
  • Flow Cytometry 1:400
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
This ABCA1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence (PMID: 21501868), Immunoprecipitation (PMID: 21846716; 21106522), Western blot and Immunohistochemistry on frozen and paraffin embedded sections. In Western blot a band representing ABCA1 is observed at ~220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal. NOTE: It is important not to boil the sample before loading onto a gel. Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter the gel. Chromatin Immunoprecipitation and ELISA were reported in scientific literature.
Background Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.
Concentration 1 mg/ml
Storage Store at 4°C. Do not freeze.
Format
Purification:
Immunogen affinity purified
Buffer System:
Tris-citrate/phosphate, pH 7-8
Preservatives:
0.02% Sodium Azide
Purity:
Affinity purified
State:
Aff - Purified
Specificity
Specificity:
This is specific for ABCA1.
Species:
Human, Mouse, Rat, Canine, Chicken, Hamster, Mustelid, Porcine.
Gene ID 19

Accessory Products

Proteins and/or Positive Controls

Proteins for ABCA1 (2 products)

Catalog No. Species Pres. Purity   Source  

ABCA1

ABCA1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ABCA1

ABCA1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.
  • LinkedIn