AP09395PU-S ABCB5 antibody

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25 µl / €190.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human, Monkey ABCB5

Product Description for ABCB5

Rabbit anti Human, Monkey ABCB5.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for ABCB5

Product Category Primary Antibodies
Target Category
Quantity 25 µl
Synonyms ATP-binding cassette sub-family B member 5
Presentation Aff - Purified
Reactivity Hu, Mky
Applications E, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q2M3G0
Immunogen:
Synthetic peptide corresponding to residues corresponding to an internal region of Human ABCB5
GeneID:
340273
Application ELISA: 1/10,000-1/50,000.
Western Blot: 1/20,000.

Trial Size:
ELISA: 1/10,000- 1/15,000.
Western Blot: 1/10,000.
Background ATP-Binding Cassette proteins comprise a superfamily of transporters with a wide variety of substrates. ABCB5 is a novel member of the human P-glycoprotein family. It functions as a determinant of membrane potential and regulator of cell fusion in physiologic skin progenitor cells. Cell fusion is thought to contribute to tissue differentiation. ABCB5 is predominantly expressed in melanoma cells and is a novel molecular marker for a distinct subset of chemoresistant stem cell phenotype-expressing tumor cells among human epidermal melanocytes. It has been identified as a novel drug transporter in human malignant melanoma. ABCB5 is a rhodamine efflux transporter. This antibody product is intended to be used to confirm cellular localization and expression level of ABCB5.
Concentration 1.29 mg/ml (AP09395PU-N) / 0.31 mg/ml (AP09395PU-S) - by UV absorbance at 280 nm
General Readings
  1. Frank NY, Margaryan A, Huang Y, Schatton T, Waaga-Gasser AM, Gasser M, et al. ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma. Cancer Res. 2005 May 15;65(10):4320-33. PubMed PMID: 15899824.
  2. Chen KG, Szakács G, Annereau JP, Rouzaud F, Liang XJ, Valencia JC, et al. Principal expression of two mRNA isoforms (ABCB 5alpha and ABCB 5beta ) of the ATP-binding cassette transporter gene ABCB 5 in melanoma cells and melanocytes. Pigment Cell Res. 2005 Apr;18(2):102-12. PubMed PMID: 15760339.
  3. Chen KG, Gottesman MM. Useful tool to generate unidirectional deletion vectors by utilizing the star activity of BamHI in an NcoI-BamHI-XhoI cassette. Biotechniques. 2005 Feb;38(2):198, 200, 202, 204. PubMed PMID: 15727125.
  4. Huang Y, Anderle P, Bussey KJ, Barbacioru C, Shankavaram U, Dai Z, et al. Membrane transporters and channels: role of the transportome in cancer chemosensitivity and chemoresistance. Cancer Res. 2004 Jun 15;64(12):4294-301. PubMed PMID: 15205344.
  5. Frank NY, Pendse SS, Lapchak PH, Margaryan A, Shlain D, Doeing C, et al. Regulation of progenitor cell fusion by ABCB5 P-glycoprotein, a novel human ATP-binding cassette transporter. J Biol Chem. 2003 Nov 21;278(47):47156-65. Epub 2003 Sep 7. PubMed PMID: 12960149.
  6. Taipalensuu J, Törnblom H, Lindberg G, Einarsson C, Sjöqvist F, Melhus H, et al. Correlation of gene expression of ten drug efflux proteins of the ATP-binding cassette transporter family in normal human jejunum and in human intestinal epithelial Caco-2 cell monolayers. J Pharmacol Exp Ther. 2001 Oct;299(1):164-70. PubMed PMID: 11561076.
Storage Store the antibody at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Storage Conditions for Trial Size:
This vial contains a relatively low volume of reagent (25 µl). To minimize loss of volume dilute 1:10 by adding 225 µl of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Shelf life: 3 month from despatch.
Format
Purification:
Affinity Chromatography
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (expected):
Monkey (85%), Rat (68%), Mouse (62%).
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody is specific for ABCB5 protein.
Gene ID 340273

Accessory Products

Proteins and/or Positive Controls

Positive controls for ABCB5 (1 products)

Catalog No. Species Pres. Purity   Source  

ABCB5 Lysate

ABCB5 Lysate [NBL1-07174] Protein
  Novus Biologicals Inc.
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