DM3017 Abl (c-Abl) antibody

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Mouse anti Human, Mouse Abl (c-Abl) 8E9


Product Description for Abl (c-Abl)

Mouse anti Human, Mouse Abl (c-Abl) 8E9.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for Abl (c-Abl)

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms AB3227, cAbl
Presentation Purified
Reactivity Hu, Ms
Applications ICC/IF, IP, P, WB
Clonality Monoclonal
Clone 8E9
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Recombinant Abl protein
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Immunofluorescence. Immunoprecipitation: Use Protein G; Ab 2µg/mg protein lysate. Western Blotting: Ab 1µg/ml for 2hrs at RT. Immunohistology: Formalin/paraffin; Use Ab 2-4µg/ml for 30min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0 for 10-20 min followed by cooling at RT for 20 min. Recommended positive control: HeLa Cells or Placenta. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.2 mg/ml
General Readings
  1. Whang YE, et al. Proc Natl Acad Sci U S A 2000 May 9; 97 (10): 5486-91.
  2. Sun
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.

Aliquoting Instructions: Do not dilute the entire reconstituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
The c-Abl proto-oncogene encodes a protein tyrosine kinase that is located in the cytoplasm and nucleus. In chronic myelogenous leukemia and in a subset of acute lymphoblastic leukemias, the c-Abl proto-oncogene undergoes a (9;22) chromosomal translocation producing a novel rearranged chromosome (The Philadelphia Chromosome) as the result of the fusion of c-Abl sequences from Chromosome 9 to the Bcr gene on Chromosome 22. c-Abl reacts with c-Abl p120 and chimeric Bcr/Abl proteins. Reacts with Human and Mouse. Others not tested. Cellular Localization: cytoplasmic, nuclear.

Mol. Wt. of Antigen: 120kD

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