AM33097PU-N Acetylated Lysine antibody

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Mouse anti All Species Acetylated Lysine 15G10


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  • AM33097PU-N

Product Description for Acetylated Lysine

Mouse anti All Species Acetylated Lysine 15G10.
Presentation: Aff - Purified
Product is tested for Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Acetylated Lysine

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms AcK, acetyl Lysine, acetyl-Lysine
Presentation Aff - Purified
Reactivity All Species
Applications ICC/IF, IP, WB
Clonality Monoclonal
Clone 15G10
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Acetylated protein mixture.
Application Western blot: Each lot of this antibody is quality control tested.
Recommended Dilutions: Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. 
Immunoprecipitation: 1/10-1/500.
Immunocytochemistry/Immunofluorescence: 1-4 µg/ml.
See also Application Reference 1.
Background Proteins are reversibly and dynamically acetylated on the ε-amino group of lysine by acetyltransferases and deacetylated by deacetylases. This post-translational modification can regulate protein function (interactions with other proteins and DNA binding). Histones and transcription factors (PCAF, p53, p300, etc) appear to be the major targets of acetytransferases. Acetylation is usually associated with chromatin remodeling and transcriptional activation, although in some cases (telomeres) it is associated with gene silencing. 
Structure: Proteins are acetylated by the reversible transfer of acetyl-CoA to the ε-amino group of lysine.
Function: Post-translational modification of proteins by acetyltransferases and deacetylated by deacetylases to regulate protein-protein and protein-DNA interactions.
Concentration 0.5 mg/ml
General Readings
  1. Koen YM, Sarma D, Hajovsky H et al. Protein Targets of Thioacetamide Metabolites in Rat Hepatocytes Chem Res Toxicol 2013 Mar 20 [PMID: 23465048] (WB)
  2. Wang S, Yan-Neale Y, Zeremski M, Cohen D. Transcription regulation by histone deacetylases. Novartis Found Symp. 2004;259:238-45; discussion 245-8, 285-8. PubMed PMID: 15171258.
  3. Legube G, Trouche D. Regulating histone acetyltransferases and deacetylases. EMBO Rep. 2003 Oct;4(10):944-7. PubMed PMID: 14528264. (Free PMC Article available, 5 images available)
  4. Chen LF, Greene WC. Regulation of distinct biological activities of the NF-kappaB transcription factor complex by acetylation. J Mol Med (Berl). 2003 Sep;81(9):549-57. Epub 2003 Aug 15. PubMed PMID: 12920522.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Affinity Chromatography
Buffer System:
PBS, pH 7.2
0.09% Sodium Azide
Liquid purified IgG fraction
Aff - Purified
The 15G10 monoclonal antibody recognizes Acetylated Lysine residues on proteins.

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