AP21365AF-N Acid Phosphatase antibody

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10 mg / €230.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Acid Phosphatase

AP21365AF-N

Product Description for Acid Phosphatase

Rabbit anti Acid Phosphatase.
Presentation: Azide Free
Product is tested for Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Properties for Acid Phosphatase

Product Category Primary Antibodies
Target Category
Quantity 10 mg
Synonyms PAP1, Purple acid phosphatase 1
Presentation Azide Free
Applications Dot, E, ICC/IF, ID, IP, R, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q6J5M7
Immunogen:
Acid Phosphatase isolated and purified from Potato.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Application This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of
the customer’s own choice.
Working dilutions in non-precipitating antibody-binding techniques: 1/1,000-1/7,000.
Background Prostatic acid phosphatase is one of the two antigenic markers of prostatic carcinoma, the other being prostate specific antigen. It belongs to the kallikrein family of serine proteases and is suggested to act as a hydrolase to split phospharyl choline in semen and as a transferase.
Concentration 10.0 mg/ml
Storage Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Shelf life: one year from despatch.
Format
Purification:
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Buffer System:
PBS, pH 7.2 without preservatives and foreign proteins.
State:
Lyophilized hyperimmune IgG fraction.
Azide Free
Reconstitution:
Restore by adding 1.0 ml of sterile distilled water
Specificity
Specificity:
Acid Phosphatase from Potato.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Species:
Potato.

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