discontinued

SP6218P Acinus (N-term of p17) antibody

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50 µg / €210.00

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Rabbit anti Human, Mouse Acinus (N-term of p17)

SP6218P

Product Description for Acinus (N-term of p17)

Rabbit anti Human, Mouse Acinus (N-term of p17).
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for Acinus (N-term of p17)

Product Category Primary Antibodies
Quantity 50 µg
Synonyms Apoptotic chromatin condensation inducer in the nucleus
Presentation Aff - Purified
Reactivity Hu, Ms
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer AnaSpec

Datasheet Extract

Immunogen
Immunogen:
Rabbit anti-Acinus (CP) polyclonal antibody was raised against a synthetic peptide corresponding to amino acids 1065 to 1080 of human AcinusL, 338 to 353 of human AcinusS', or 307 to 322 of human AcinusS, which are identical to those of mouse Acinus (2). The selected antigenic sequence is located near the C-terminus of the cleaved active peptide p17.
Application Western blot: 0.5 - 1 µg/ml. K562 cell lysate can be used as positive control and an approximate 220 kDa band can be detected. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.5 mg/ml
General Readings
  1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature. 1999 Sep 9;401(6749):127-8. PubMed PMID: 10490018.
  2. Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y. Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Nature. 1999 Sep 9;401(6749):168-73. PubMed PMID: 10490026.
Storage Store the antibody at 4-8°C for one month or at -20°C for longer. This product should be stored undiluted. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format
State:
Aff - Purified
Specificity
Specificity:
This antibody recognises Acinus (apoptosis chromatin condensation inducer in the nucleus), an inducer of chromatin condensation.
Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE (1). A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS' (2).

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