AP20312PU-N ACO1 / IREB1 antibody

See related secondary antibodies

Search for all "ACO1 / IREB1"

0.1 mg / €350.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human, Mouse, Rat ACO1 / IREB1

Product Description for ACO1 / IREB1

Rabbit anti Human, Mouse, Rat ACO1 / IREB1.
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for ACO1 / IREB1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Aconitase, Citrate hydro-lyase, Cytoplasmic aconitate hydratase, Ferritin repressor protein, IRP1, Iron regulatory protein 1, Iron-responsive element-binding protein 1
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications P, WB
Clonality Polyclonal
Host Rabbit
Molecular weight ~ 98 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P21399
GeneID:
48
Application Immunohistochemistry on paraffin sections: 1/50-1/200.
Background The insulin receptor substrate-1 (IRS-1), a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor. The association of 14-3-3 with IRS-1 increases significantly upon treatment with okadaic acid, a potent serine/ threonine phosphatase inhibitor.
Concentration 1.0 mg/ml
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity-chromatography using epitope-specific immunogen; purity is > 95% (by SDS-PAGE)
Buffer System:
Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (expected):
Mouse and Rat.
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody detects endogenous levels of IRP-1 protein.
Gene ID 48

Accessory Products

Proteins and/or Positive Controls

Proteins for ACO1 / IREB1 (12 products)

Catalog No. Species Pres. Purity   Source  

ACO1 / IREB1

ACO1 / IREB1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ACO1 / IREB1 (1-889, His-tag)

ACO1 / IREB1 Human Purified > 90 % E. coli
50 µg / €750.00
  Acris Antibodies GmbH

ACO1 / IREB1 (1-889, His-tag)

ACO1 / IREB1 Human Purified > 90 % E. coli
10 µg / €300.00
  Acris Antibodies GmbH

ACO1 / IREB1

ACO1 / IREB1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

ACO1 / IREB1

ACO1 / IREB1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

ACO1 / IREB1

ACO1 / IREB1 Human Purified
  Abnova Taiwan Corp.

ACO1 / IREB1

ACO1 / IREB1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

ACO1 / IREB1

ACO1 / IREB1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

ACO1 / IREB1

ACO1 / IREB1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

ACO1 / IREB1

ACO1 / IREB1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

ACO1 / IREB1

ACO1 / IREB1 Human Purified
  Novus Biologicals Inc.

ACO1 / IREB1

ACO1 / IREB1 Human Purified
  Novus Biologicals Inc.

Positive controls for ACO1 / IREB1 (2 products)

Catalog No. Species Pres. Purity   Source  

ACO1 293T Cell Transient Overexpression Lysate(Denatured)

ACO1 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

Aconitase 1 Lysate

Western Blot: Aconitase 1 Lysate [NBL1-07243] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for ACO1 Protein
  Novus Biologicals Inc.
  • LinkedIn