AP31817PU-N ACPP / Prostatic acid phosphatase (Pain System Marker) antibody

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0.2 ml / €360.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Chicken anti Mouse ACPP / Prostatic acid phosphatase

Product Description for ACPP / Prostatic acid phosphatase

Chicken anti Mouse ACPP / Prostatic acid phosphatase.
Properties: (Pain System Marker)
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Properties for ACPP / Prostatic acid phosphatase

Product Category Primary Antibodies
Target Category
Quantity 0.2 ml
Synonyms ACP3, PAP, PSAP, Prostate acid phosphatase
Presentation Aff - Purified
Reactivity Ms
Applications ICC/IF, P
Clonality Polyclonal
Host Chicken
Isotype Ig
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Recombinant Mouse PAP protein was expressed using a baculoviral-delivery system.
Preparation: After repeated injections, immune eggs were collected from laying hens, from which IgY antibody were prepared (“anti-PAP IgY fraction”). Some of this antibody was further purified using an agarose matrix to which the PAP protein was convalently attached (“Affinity-purified anti-PAP”). The final preparation in the accompanying vial contains 10 mg/ml of the “anti-PAP IgY fraction” supplemented with 20 mg/ml of the “affinity-purified anti-PAP” plus 50% (v/v) Glycerol (to prevent freezing at –20°C). Finally, this antibody preparation was filter-sterilized (0.45 mm) and 200 µl aliquots prepared.
Property Pain System Marker
Application Immunocytochemistry.
Immunohistochemistry (1/500-1/1000).
Quality Control: Antibodies were analyzed using immunohistochemistry with tissue sections through a 10%-formalin fixed adult Mouse. Sections were examined for PAP-positive dorsal root ganglion sensory neurons. Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) used as the secondary reagent.
Background Mouse PAP is a 43,698 dalton protein (381 amino acids; NCBI accession numberAAF23171) associated with prostatic cancer cells, as well as primary afferent sensory neurons involved in the pain pathway. This protein is an enzyme that dephosphorylates adenosine monophosphate (AMP) in the dorsal horn gray matter of the spinal cord, generating free adenosine. Injections of PAP into the dorsal horn of experimental mice has been shown to decrease pain perception by acting in an antinociceptive, antihyperalgesic, and antiallodynic fashion.
Concentration 10 mg IgY/ml (based on absorbance at 280 nm)
Storage Store the antibody undiluted in the dark at 2-8°C.
Shelf life: one year from despatch.
Affinity Chromatography using a peptide column.
Buffer System:
10mM PBS, pH 7.2 containing 1% BSA as stabilizer and 0.02% Sodium Azide as preservative.
Liquid purified (filter sterilized) IgY fraction.
Aff - Purified
Recognizes Mouse Prostatic Acid Phosphatase (PAP).
Gene ID 56318

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