AP32845PU-S Actin antibody

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0.1 ml / €180.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Chicken anti Rabbit Actin


Product Description for Actin

Chicken anti Rabbit Actin.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for Actin

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Aff - Purified
Reactivity Rb
Applications WB
Clonality Polyclonal
Host Chicken
Isotype Ig
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Chickens were immunized with actin purified from Rabbit muscle.
After epeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column. The final product contains 50% glycerol (v/v), purified anti-Actin IgY fraction (10 mg/ml) spiked with 25 µg/ml of affinity purified anti-Actin IgY, and then filter-sterilized.
Application Quality Control: This anti-Actin preparation was validated by Western blotting on nitrocellulose membranes (at a concentration of 1 µg/ml) using HRP-Conjugated Goat anti-Chicken IgY (1/5000 dilution, Cat.-No AP31795HR-N) as the secondary reagent.
Recommended Dilutions: 1/1000-1/2000 for Western blot analysis of Nitrocellulose membranes.
Please note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.
Do Not use this product with PVDF, or other nylon-based membranes, because this often produces unacceptably high backgrounds in chemiluminescence studies.
Concentration 10 mg/ml of purified anti-Actin (purified from rabbit muscle) IgY spiked with 25 ug/ml of affinity purified anti-Actin IgY (based on A280 nm assay readings)
Storage Store undiluted at -20°C
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Affinity Chromatography
Buffer System:
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2)
0.02% (w/v) Sodium Azide
Liquid purified IgY fraction
Aff - Purified

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