SM1349P Actin (F-Actin) antibody

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0.5 mg / €440.00
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Quick Overview

Mouse anti Human, Mouse, Rabbit, Rat Actin (F-Actin) NH3

SM1349P

Product Description for Actin (F-Actin)

Mouse anti Human, Mouse, Rabbit, Rat Actin (F-Actin) NH3.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Enzyme Immunoassay, Frozen Sections, Flow Cytometry.

Properties for Actin (F-Actin)

Product Category Primary Antibodies
Quantity 0.5 mg
Synonyms Actin F type
Presentation Purified
Reactivity Hu, Ms, Rb, Rt
Applications C, E, F, WB
Clonality Monoclonal
Clone NH3
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Human monocytes and U937 cell line.
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Application ELISA: 1/10.
Western Blotting: 1/100-1/500.
Flow Cytometry: 
1/10
Immunohistochemistry on Frozen Sections.
Background Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. G-Actin (globular actin) with bound ATP can polymerise to form F-Actin (Filamentious Actin). Actin monomers spiral ariound the axis of the filament similar to a double helix.
F-Actin may also undergo a process called treadmilling, in which filament length remains constant and actin monomers add at one end and dissociated at the other.  
Concentration 1.0 mg/ml
General Readings
  1. Dransfield, I. et al. (1988) Initial characterization of an anti-actin monoclonal antibody (NH3). Biochem. Soc. Trans. 16: 163-164.
  2. McCarthy AM, Spisak KO, Brozinick JT, Elmendorf JS. Loss of cortical actin filaments in insulin-resistant skeletal muscle cells impairs GLUT4 vesicle trafficking and glucose transport. Am J Physiol Cell Physiol. 2006 Nov;291(5):C860-8. Epub 2006 Jun 14. PubMed PMID: 16774991. (Free PMC Article available, 6 images available)
  3. Bhonagiri P, Pattar GR, Habegger KM, McCarthy AM, Tackett L, Elmendorf JS. Evidence coupling increased hexosamine biosynthesis pathway activity to membrane cholesterol toxicity and cortical filamentous actin derangement contributing to cellular insulin resistance. Endocrinology. 2011 Sep;152(9):3373-84. doi: 10.1210/en.2011-1295. Epub 2011 Jun 28. PubMed PMID: 21712361. (Free PMC Article available, 8 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgM fraction prepared from Tissue Culture Supernatant
Purified
Specificity
Specificity:
This antibody recognizes Human Filamentous Actin (F-actin).
The antibody binds to the N-terminal region of Actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types.
Clone NH3 is reported to recognize Actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule.
Clone NH3 is also described to show reactivity with a 43 kDa polypeptide using cell lines U937 and HL-60 by SDS/PAGE and Immunoblotting.
Species:
Human, Rat, Mouse and Rabbit.
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