AM00527PU-N Actin (pan alpha) antibody

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Mouse anti Bovine, Canine, Equine, Human, Mouse, Porcine, Rat Actin (pan alpha) MSA06

Product Description for Actin (pan alpha)

Mouse anti Bovine, Canine, Equine, Human, Mouse, Porcine, Rat Actin (pan alpha) MSA06.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Properties for Actin (pan alpha)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Bov, Can, Eq, Hu, Ms, Por, Rt
Applications ICC/IF, P
Clonality Monoclonal
Clone MSA06
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Purified Actin
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Immunofluorescence.
Immunohistology (Formalin/Paraffin): Use Ab at 1-2 µg/ml for 30 min at RT. (no special pretreatment is required for immunohistochemical staining of formalin-fixed tissues).
Recommended Positive Control: Muscle or sarcoma.
Background Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Concentration 0.2 mg/ml
General Readings
  1. Tsukada T. et al. Am J Pathol 1987, 126:51.
  2. Tsukada T. et. al. Am J Pathol 1987, 127:389.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein G Chromatography.
Buffer System:
10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% Sodium Azide.
Liquid purified Ig fraction.
This antibody reacts with alpha-smooth muscle as well as alpha-skeletal and alpha-cardiac (sarcomeric) isoform of Actin. This pan muscle Actin antibody reacts with tumors arising from smooth muscle (leiomyosarcomas) as well as skeletal muscle (rhabdomyosarcomas).
Cellular Localization: Cytoplasmic
Human, Mouse, Rat, Porcine, Horse, Bovine, Canine.
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